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APC Mouse Anti-Human CD45RO
商品详情
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BD™
CD45R; PTPRC; LCA; Leukocyte common antigen; GP180; LY5; T200
Human
Mouse BALB/c IgG2a, κ
Human IL-2-dependent T-cell line
Flow cytometry
50 μg/mL
5 μL
IV N31
5788
Phosphate buffered saline with gelatin and 0.1% sodium azide.
RUO (GMP)


准备和存储

The PE conjugate is supplied as 12.5 μg in 2.0 mL (6.25 μg/mL) of phosphate buffered saline (PBS). The PE-Cy7 conjugate is supplied as 25 μg in 0.5 mL (50 μg/mL) of PBS. The APC conjugate is supplied as 25 μg in 0.5 mL (50 μg/mL) of PBS. PBS contains gelatin and 0.1% sodium azide.

Store vials at 2° to 8°C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.

340438 Rev. 1
抗体详情
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UCHL1

The CD45RO antibody, clone UCHL-1, is derived from hybridization of mouse P3/NS-1/1-Ag4-1 myeloma cells with spleen cells from BALB/c mice immunized with an interleukin-2 (IL-2)–dependent human T-cell line.

The CD45RO antibody recognizes a 180-kilodalton (kDa) isoform of the leucocyte common antigen (LCA). CD45 is a member of the CD45 family that includes CD45, CD45RA, and CD45RB. The CD45 antigen is a protein tyrosine phosphatase.

340438 Rev. 1
格式详情
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APC
Allophycocyanin (APC), is part of the BD family of phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 651 nm and an emission maximum (Em Max) at 660 nm. APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
APC
Red 627-640 nm
651 nm
660 nm
340438 Rev.1
报价单和参考
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View product citations for antibody "340438" on CiteAb

研发参考 (10)

  1. Akbar AN, Terry L, Timms A, Beverley PC, Janossy G. Loss of CD45R and gain of UCHL1 reactivity is a feature of primed T cells. J Immunol. 1988; 140(7):2171-2178. (Biology). 查看参考
  2. D'Arena G, Cascavilla N, Nunziata G, et al. Usefulness of RA and RO isoforms of common leukocyte antigen (CD45) for early distinction between normal and abnormal promyelocytes. Leuk Lymphoma. 2002; 43:1823-1825. (Biology).
  3. Gamadia LE, Rentenaar RJ, Baars PA, et al. Differentiation of cytomegalovirus-specific CD8(+) T cells in healthy and immunosuppressed virus carriers.. Blood. 2001; 98(3):754-61. (Biology). 查看参考
  4. Ledbetter JA, Tonks NK, Fischer EH, Clark EA. CD45 regulates signal transduction and lymphocyte activation by specific association with receptor molecules on T or B cells. Proc Natl Acad Sci USA. 1988; 85:8628-8632. (Biology).
  5. Norton AJ, Ramsay AD, Smith SH, Beverley PC, Isaacson PG. Monoclonal antibody (UCHL1) that recognises normal and neoplastic T cells in routinely fixed tissues. J Clin Pathol. 1986; 39(4):399-405. (Biology). 查看参考
  6. Ostrowski SR, Gerstoff J, Pederson BK, Ullum H. A low level of CD4+CD28+ T cells is an independent predictor of high mortality in human immunodeficiency virus type 1–infected patients. J Infec Dis. 2003; 187:1726-1734. (Biology).
  7. Picker LJ, Treer JR, Ferguson-Darnell B, Collins PA, Buck D, Terstappen LW. Control of lymphocyte recirculation in man. I. Differential regulation of the peripheral lymph node homing receptor L-selectin on T cells during the virgin to memory cell transition. J Immunol. 1993; 150(3):1105-1121. (Biology). 查看参考
  8. Smith SH, Brown MH, Rowe D, Callard RE, Beverley PC. Functional subsets of human helper-inducer cells defined by a new monoclonal antibody, UCHL-1. Immunology. 1986; 58:63-70. (Biology).
  9. Streuli M, Morimoto C, Schrieber M, Schlossman SF, Saito H. Characterization of CD45 and CD45R monoclonal antibodies using transfected mouse cell lines that express individual human leukocyte common antigens. J Immunol. 1988; 141(11):3910-3914. (Biology). 查看参考
  10. Tonks NK, Charbonneau H, Diltz CD, Fischer EH, Walsh KA. Demonstration that the leukocyte common antigen CD45 is a protein tyrosine phosphatase. Biochemistry. 1988; 27:8695-8701. (Biology).
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340438 Rev. 1

 

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