Skip to main content Skip to navigation
Hamburger Menu
Search icon
Country Selector Country Selector
China (Chinese)
产品

解决方案

发现&学习

资源&工具

Support

Search icon Search icon
Close Menu
      Alert Icon
      APC Mouse Anti-Human CD117
      商品详情
      Down Arrow Up Arrow


      BD™
      KIT; c-Kit; SCFR; PBT; Mast/stem cell growth factor receptor
      Human
      Mouse BALB/c IgG1
      Megakaryoctic cell line MOLM-1
      Flow cytometry
      10 μg/mL
      5 μL
      VI C30
      3815
      AB_400563
      Phosphate buffered saline with gelatin and 0.1% sodium azide.
      RUO (GMP)


      准备和存储

      The PE conjugate is supplied as 10 µg in 1.0 mL (10 µg/mL) of phosphate-buffered saline (PBS). The PerCP-Cy5.5 conjugate is supplied as 1 µg in 1.0 mL (1 µg/mL) of PBS. The PE-Cy7 conjugate is supplied as 6.25 µg in 0.5 mL (12.5 µg/mL) of PBS. The APC conjugate is supplied as 5 µg in 0.5 mL (10 µg/mL) of PBS. PBS contains gelatin and 0.1% sodium azide.

      Store vials at 2°C–8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.

      显示较多信息 blue down arrow 显示较少信息 blue up arrow
      341096 Rev. 1
      抗体详情
      Down Arrow Up Arrow
      104D2

      The CD117 antibody, clone 104D2, is derived from the hybridization of Sp2/0 mouse myeloma cells with spleen cells isolated from BALB/c mice immunized with the megakaryocytic cell line MOLM-1.

      The CD117 antibody binds to a 145-kilodalton (kDa) type I transmembrane glycoprotein in the receptor tyrosine kinase (RTK) family. The CD117 antigen is also known as c-kit and stem cell factor receptor (SCFR).

      341096 Rev. 1
      格式详情
      Down Arrow Up Arrow
      APC
      Allophycocyanin (APC), is part of the BD family of phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 651 nm and an emission maximum (Em Max) at 660 nm. APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      APC
      Red 627-640 nm
      651 nm
      660 nm
      341096 Rev.1
      报价单和参考
      Down Arrow Up Arrow
      View product citations for antibody "341096" on CiteAb

      研发参考 (21)

      1. Ashman LK, Cambareri A, Nguyen L, Bühring H-J. CD117 workshop panel report. In: Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997:816-818.
      2. Berardi AC, Wang A, Levine JD, Lopez P, Scadden DT. Functional isolation and characterization of human hematopoietic stem cells. Science. 1995; 267:104-108. (Biology). 查看参考
      3. Briddell RA, Broudy VC, Bruno E, Brandt JE, Srour EF, Hoffman R. Further phenotypic characterization and isolation of human hematopoietic progenitor cells using a monoclonal antibody to the c-kit receptor. Blood. 1992; 79:3159-3167. (Biology). 查看参考
      4. Broudy VC, Lin N, Zsebo KM, et al. Isolation and characterization of a monoclonal antibody that recognizes the human c-kit receptor. Blood. 1992; 79:338-346. (Biology). 查看参考
      5. Centers for Disease Control. Perspectives in disease prevention and health promotion update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in health-care settings. MMWR. 1988; 37:377-388. (Biology).
      6. Clinical and Laboratory Standards Institute. 2005. (Biology).
      7. Deberry C, Mou S, Linnekin D. Stat1 associates with c-kit and is activated in response to stem cell factor. Biochem J. 1997; 32773-80. (Biology).
      8. Gunji Y, Nakamura M, Osawa H, et al. Human primitive hematopoietic progenitor cells are more enriched in KITlow cells than in KIThigh cells. Blood. 1993; 3283-3289. (Biology).
      9. Hashimoto K, Matsumura I, Tsujimura T, et al. Necessity of tyrosine 719 and phosphatidylinositol 3'-kinase-mediated signal pathway in constitutive activation and oncogenic potential of c-kit receptor tyrosine kinase with the Asp814Val mutation. Blood. 2003; 101:1094-1102. (Biology).
      10. Ikuta K, Weissman IL. Evidence that hematopoietic stem cells express mouse c-kit but do not depend on steel factor for their generation. Proc Natl Acad Sci U S A. 1992; 89(4):1502-1506. (Biology). 查看参考
      11. Kinashi T, Springer TA. Steel factor and c-kit regulate cell-matrix adhesion. Blood. 1994; 83:1033-1038. (Biology). 查看参考
      12. Maddens S, Charruyer A, Plo I, et al. Kit signaling inhibits the sphingomyelin-ceramide pathway through PLCγ1: implication in stem cell factor radioprotective effect. Blood. 2002; 100:1294-1301. (Biology).
      13. Meininger CJ, Yano H, Rottapel R, Bernstein A, Zsebo KM, Zetter BR. The c-kit receptor ligand functions as a mast cell chemoattractant. Blood. 1992; 79:958-963. (Biology).
      14. Nocka K, Buck J, Levi E, Besmer P. Candidate ligand for the c-kit transmembrane kinase receptor: KL, a fibroblast derived growth factor stimulates mast cells and erythroid progenitors. EMBO J. 1990; 9:3287-3294. (Biology).
      15. Nocka K, Majumder S, Chabot B, et al. Expression of c-kit gene products in known cellular targets of W mutations in normal and W mutant mice- evidence for an impaired c-kit kinase in mutant mice. Genes Dev. 1989; 3:816-826. (Biology).
      16. Okada S, Nakauchi H, Nagayoshi K, Nishikawa S, Miura Y, Suda T. Enrichment and characterization of murine hematopoietic stem cells that express c-kit molecule. Blood. 1991; 78:1706-1712. (Biology). 查看参考
      17. Ratajczak MZ, Luger SM, DeRiel K, Abrahm J, Calabretta B, Gewirtz AM. Role of the KIT protooncogene in normal and malignant human hamatopoiesis. Proc Natl Acad Sci USA. 1992; 89:1710-1714. (Biology).
      18. Samayawardhena LA, Kapur R, Craig AW. Involvement of Fyn kinase in Kit and integrin-mediated Rac activation, cytoskeletal reorganization, and chemotaxis of mast cells. Blood. 2007; 109:3679-3686. (Biology).
      19. Simmons P, Aylett G, Niutta S, To L, Juttner C, Ashman L. c-kit is expressed by primitive human hematopoietic cells that give rise to colony-forming cells in stroma-dependent or cytokinesupplemented culture. Exp Hematol. 1994; 22:157-165. (Biology).
      20. Ueda S, Mizuki M, Ikeda H, et al. Critical roles of c-Kit tyrosine residues 567 and 719 in stem cell factor-induced chemotaxis: contribution of src family kinase and PI3-kinase on calcium mobilization and cell migration. Blood. 2002; 99:3342-3349. (Biology).
      21. Zola H, Swart B, Nicholson I, Voss E. Leukocyte and Stromal Cell Molecules: The CD Markers. 2007. (Biology).
      查看所有文件 (21) 查看更少内容
      341096 Rev. 1

       

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures. 

       

      Although not required, these products are manufactured in accordance with Good Manufacturing Practices.