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Antibody Cocktail (IL-2 FITC, CD69 PE, CD4 PerCP-Cy™5.5, CD3 APC)

BD FastImmune™ Antibody Cocktail (IL-2 FITC, CD69 PE, CD4 PerCP-Cy™5.5, CD3 APC)

(RUO (GMP))
商品详情
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BD FastImmune™
Human
Intracellular staining (flow cytometry)
RUO (GMP)
Phosphate buffered saline with BSA, beta-lactoglobulin, and 0.1% sodium azide.


描述

Anti-Hu–IL-2, clone 5344.111, is derived from a fusion of Sp2/0 myeloma cells with splenocytes from BALB/c mice immunized with recombinant human IL-2.

CD69, clone L78, is derived from hybridization of mouse Sp2/0-Ag14 myeloma cells with lymph node cells from BALB/c mice immunized with a CD8+ alloantigen-directed cytotoxic T-lymphocyte (CTL) cell line.

CD4, clone SK3, is derived from hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immununized with human peripheral blood T lymphocytes.

CD3, clone SK7, is derived from hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with human thymocytes.

Anti-Human Interleukin-2 (Anti-Hu–IL-2) recognizes a 15- to 18-kilodalton (kd) glycoprotein.

CD69 recognizes a very early human activation antigen. The CD69 antigen is a surface homodimer formed by the association of 28-kd and 32-kd chains that are held together by disulfide bridges.

The CD4 antigen, Mr 55 kd, is present on T-helper/inducer lymphocytes and monocytes.

CD3 reacts with the epsilon chain of the CD3 antigen/T-cell antigen receptor (TCR) complex. This complex is composed of at least six proteins that range in molecular weight from 20 to 30 kd. The antigen recognized by CD3 antibodies is noncovalently associated with either α/β or γ/δ TCR (70 to 90 kd).

准备和存储

Store vials at 2° to 8°C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.

337188 Rev. 1
组件
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描述 克隆 同型对照 EntrezGene ID
CD3 APC SK7 IgG1, κ N/A
IL-2 FITC 5344.111 IgG1, κ N/A
CD69 PE 2D7/Basophils IgG1, κ N/A
CD4 PerCP-CY5.5 SK3 IgG1, κ N/A
337188 Rev. 1
报价单和参考
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研发参考 (45)

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  39. Testi R, Philips JH, Lanier LL. Leu-23 induction as an early marker for functional CD3/T cell antigen receptor triggering: requirement for receptor cross-linking, prolonged elevation of intracellular (Ca ++ ), and stimulation of protein kinase C. J Immunol. 1988; 2:1214. (Biology).
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  41. Testi R, Pulcinelli F, Frati L, Gazzaniga PP, Santoni A. CD69 is expressed on platelets and mediates platelet activation and aggregation.. J Exp Med. 1990; 172(3):701-7. (Biology). 查看参考
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337188 Rev. 1

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