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L-selectin; LECAM-1; SELL; LNHR; LSEL; LAM-1; LEU8; PLNHR; TQ-1; MEL-14
Mouse BALB/c IgG2a, κ
Human Peripheral Blood T Lymphocytes
Flow cytometry
10 μg/mL
20 μL
V S059

654666 Rev. 1
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CD62L, clone SK11, is derived from hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with peripheral blood T lymphocytes.

The CD62L antigen, Mr 80 kilodaltons (kd), is the leucocyte endothelial cellular adhesion molecule (LECAM). The CD62L antigen belongs to the selectin family of cell adhesion molecules. The CD62L molecule is the human homologue of the murine lymph node homing receptor, MEL 14.

654666 Rev. 1
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R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
654666 Rev.1
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研发参考 (13)

  1. Bevilacqua M, Butcher E, Furie B, et al. Selectins: a family of adhesion receptors. Cell. 1991; 67:233. (Biology).
  2. Camerini D, James SP, Stamenkovic I, Seed B. Leu-8/TQ1 is the human equivalent of the Mel-14 lymph node homing receptor. Nature. 1989; 342(6245):78-82. (Biology). 查看参考
  3. Centers for Disease Control. Update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in healthcare settings. MMWR. 1988; 37:377-388. (Biology).
  4. Clinical and Laboratory Standards Institute. 2005. (Biology).
  5. Diacovo T, Springer TA. CD62L (L-selectin) cluster report. In: Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995:1503-1504.
  6. Gatenby PA, Kansas GS, Xian CY, Evans RL, Engleman EG. Dissection of immunoregulatory subpopulations of T lymphocytes within the helper and suppressor sublineages in man. J Immunol. 1982; 129(5):1997-2000. (Biology). 查看参考
  7. Kanof ME, James SP. Leu-8 antigen expression is diminished during cell activation but does not correlate with effector function of activated T lymphocytes. J Immunol. 1988; 140:3701-3706. (Biology).
  8. Kanof ME, Strober W, James SP. Induction of CD4 suppressor T cells with anti-Leu-8 antibody. J Immunol. 1987; 139(1):49-54. (Biology). 查看参考
  9. Kansas GS, Wood GS, Engleman EG. Maturational and functional diversity of human B lymphocytes delineated with Anti-Leu-8. J Immunol. 1985; 134:3003-3006. (Biology).
  10. Kansas GS, Wood GS, Fishwild DM, Engleman EG. Functional characterization of human T lymphocyte subsets distinguished by monoclonal anti-leu-8. J Immunol. 1985; 134(5):2995-3002. (Biology). 查看参考
  11. Lanier LL, Engleman EG, Gatenby P, Babcock GF, Warner NL, Herzenberg LA. Correlation of functional properties of human lymphoid cell subsets and surface marker phenotypes using multiparameter analysis and flow cytometry. Immun Rev. 1983; 74:143. (Biology).
  12. Lanier LL, Loken MR. Human lymphocyte subpopulations identified by using three-color immunofluorescence and flow cytometry analysis: correlation of Leu-2, Leu-3, Leu-7, Leu-8, and Leu-11 cell surface antigen expression. J Immunol. 1984; 132:151. (Biology).
  13. Michie SA, Garcia CF, Strickler JG, Dailey MO, Rouse RV, Warnke RA. Expression of the Leu-8 antigen by B-cell lymphomas. Am J Clin Pathol. 1987; 88(4):486-490. (Biology). 查看参考
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654666 Rev. 1

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