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CD3-epsilon; CD3E; Leu4; T-cell surface antigen T3/Leu-4 epsilon chain; T3E
Mouse BALB/c IgG1, κ
Human Thymocytes
Flow cytometry
20 μL
II T118; III T492
Buffered saline with gelatin and 0.1% sodium azide.


1. For in vitro diagnostic use.

2. When stored at 2° to 8°C, the antibody reagent is stable until the expiration date shown on the label. Do not use after the expiration date.

3. The antibody reagent should not be frozen or exposed to direct light during storage or during incubation with cells. Keep the reagent vial dry.

4. Alteration in the appearance of the reagent, such as precipitation or discoloration, indicates instability or deterioration. In such cases, the reagent should not be used.

5. The antibody reagent contains sodium azide as a preservative; however, care should be taken to avoid microbial contamination, which may cause erroneous results.

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Becton Dickinson Immunocytometry Systems (BDIS) CD3 fluorescein isothiocyanate (FITC) reagent is a single-color direct immunofluorescence reagent for identifying and enumerating T lymphocytes using fluorescence microscopy or flow cytometers such as the FACSCalibur™, FACSort™, FACScan™, or FACS Analyzer™.

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Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Blue 488 nm
494 nm
518 nm
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研发参考 (24)

  1. Brain P, Gordon J, Willets W. Rosette formation by peripheral lymphocytes. Clin Exp Immunol. 1970; 6:681-688. (Biology).
  2. Brenner M, Groh V, Porcelli S, et al. Knapp W, Dörken B, Gilks W, et al, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. New York: Oxford University Press; 1989:1049-1053.
  3. Brown G, Greaves M. Enumeration of absolute numbers of T and B cells in human blood. Scand J Immunol. 1974; 3:161. (Biology).
  4. Centers for Disease Control. Update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in healthcare settings. MMWR. 1988; 37:377-388. (Biology).
  5. Clevers H, Alarcón B, Wileman T, Terhorst C. The T cell receptor/CD3 complex: a dynamic protein ensemble. Annual Rev Immunol. 1988; 6:629. (Biology).
  6. Dwyer J, Finklestein F, Mangi R, Fisher K, Hendler E. Assessment of the adequacy of immunosuppressive therapy using microscopy techniques to study immunologic competence. Transplant Proc. 1975; 7:785. (Biology).
  7. Freidrich W, O'Reilly R, Kozenger B, Gebhard D, Good R, Evans R. T lymphocyte reconstitution in recipients of bone marrow transplants: Analysis of regulatory T-cell imbalances in GVHD. Blood. 1982; 59:696. (Biology).
  8. Giorgi JV. Lymphocyte subset measurements: significance in clinical medicine. In: Rose NR, Friedman H, Fahey JL, ed. Manual of Clinical Laboratory Immunology. 3rd ed.. Washington, DC: American Society for Microbiology; 1986:236-246.
  9. Haynes BF. Summary of T-cell studies performed during the Second International Workshop and Conference on Human Leukocyte Differentiation Antigens. In: Reinherz EL. Ellis L. Reinherz .. et al., ed. Leukocyte typing II. New York: Springer-Verlag; 1986:3-30.
  10. Jackson AL, Warner NL. Rose NR, Friedman H, Fahey JL, ed. Manual of Clincial Laboratory Immunology, Third Edition. Washington DC: American Society for Microbiology; 1986:226-235.
  11. Jondal M, Holm G, Wigzell H. Surface markers on human T and B lymphocytes, I: A large population of lymphocytes forming nonimmune rosettes with sheep red blood cells. J Exp Med. 1972; 136:207-215. (Biology).
  12. Kan EA, Wang CY, Wang LC, Evans RL. Noncovalently bonded subunits of 22 and 28 kd are rapidly internalized by T cells reacted with anti-Leu-4 antibody. J Immunol. 1983; 131(2):536-539. (Biology). 查看参考
  13. Knowles RW. Immunochemical analysis of the T-cell–specific antigens. In: Reinherz EL. Ellis L. Reinherz .. et al., ed. Leukocyte typing II. New York: Springer-Verlag; 1986:259-288.
  14. Kohler G, Milstein C. Continuous cultures of fused cells secreting antibody of predefined specificity. Nature. 1975; 256:495. (Biology).
  15. Lanier LL, Le AM, Phillips JH, Warner NL, Babcock GF. Subpopulations of human natural killer cells defined by expression of the Leu-7 (HNK-1) and Leu-11 (NK-15) antigens. J Immunol. 1983; 131(4):1789-1796. (Biology). 查看参考
  16. Ledbetter JA, Evans RL, Lipinski M, Cunningham-Rundles C, Good RA, Herzenberg LA. Evolutionary conservation of surface molecules that distinguish T lymphocyte helper/inducer and cytotoxic/suppressor subpopulations in mouse and man. J Exp Med. 1981; 153(2):310-323. (Biology). 查看参考
  17. Mishell B, Shiigi S, Henry C, et al. Mishell B, Shiigi S, ed. Selected Methods in Cellular Immunology. New York: WH Freeman and Co; 1980:16-17.
  18. Protection of Laboratory Workers from Infectious Disease Transmitted by Blood, Body Fluids, and Tissue: Tentative Guideline. NCCLS document M29-T2. (Biology).
  19. Schroff R, Gale R, Fahey J. Regeneration of T-cell subpopulations after bone marrow transplantation: Cytomegalovirus infection and lymphoid subset imbalance. J Immunol. 1982; 129:1926. (Biology).
  20. Schroff R, Gottlieb M, Prince H, Chai L, Fahey J. Immunological studies of homosexual men with immunodeficiency and Kaposi’s sarcoma. Clin Immunol Immunopathol. 1983; 27:300-314. (Biology).
  21. Smolen J, Chused T, Leiserson W, Reeves J, Alling D, Steinberg A. Heterogeneity of immunoregulatory T-cell subsets in systemic lupus erythematosus. Am J Med. 1982; 72:783-790. (Biology).
  22. WHO/IARC. Special Technical Report; Identification, enumeration and isolation of B and T lymphocytes from human blood. Scand J Immunol. 1974; 3:521. (Biology).
  23. West W, Boozer R, Herberman R. Low affinity E-rosette formation by the human K cell. J Immunol. 1978; 120:90. (Biology).
  24. van Dongen JJM, Krissansen GW, Wolvers-Tettero ILM, et al. Cytoplasmic expression of the CD3 antigen as a diagnostic marker for immature T-cell malignancies. Blood. 1988; 71:603-612. (Biology).
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