CD27 PE-Cy™7

BD™ CD27 PE-Cy™7

Name: CD27 PE-Cy™7
Brand: BD™
SKU: 655157

克隆 M-T271

(ASR)

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科学资源

商品详情

Brand: BD™

Alternative Name: TNFRSF7; TNF receptor superfamily, member 7; T14; Tp55; S152

Reactivity: Human

Isotype: Mouse BALB/c IgG1, κ

Immunogen: Human T-CLL cells

Application: Flow cytometry

Concentration: 50 μg/mL

Vol. Per Test: 5 μL

Workshop Number: IV T187; V 5T CD27.03

Entrez Gene ID: 939

Regulatory Status: ASR

655157 Rev. 1

抗体详情

M-T271

The CD27 antibody, clone M-T271, is derived from the hybridization of mouse myeloma cells from mice immunized with human T-cell lymphocytic leukemia (T-CLL) cells.

The CD27 antibody recognizes a 55-kilodalton (kDa) disulfide-linked dimer that is a member of the nerve growth factor (NGF) superfamily, which also includes CD40, tumor necrosis factor (TNF) receptors α and β, and CD95 (Fas). With its ligand CD70, CD27 acts in a co-stimulatory fashion on T lymphocytes.

655157 Rev. 1

格式详情

PE-Cy7

PE-Cy7 dye is a part of the BD PE family of dyes. This tandem fluorochrome is comprised of a R-Phycoerythrin (PE) donor that has excitation maxima (Ex Max) of 496-nm and 566-nm and an acceptor dye, Cy™7, with an emission maximum (Em Max) at 781-nm. PE can be excited by the Blue (488-nm), Green (532-nm) and yellow-green (561-nm) lasers and detected using an optical filter centered near 781 nm (e.g., a 760/60-nm bandpass filter). The donor dye can be excited by the Blue (488-nm), Green (532-nm) and yellow-green (561-nm) lasers and the acceptor dye can be excited by the Red (627–640-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

格式: PE-Cy7

激发源: Yellow-Green 488 nm, 532 nm, 561 nm

最多激发源: 496 nm, 566 nm

最多发射源: 781 nm

655157 Rev.1

报价单和参考

研发参考 (13)

Baars PA, Maurice MM, Rep M, Hooibrink B, van Lier RA. Heterogeneity of the circulating human CD4+ T cell population. Further evidence that the CD4+CD45RA-CD27- T cell subset contains specialized primed T cells. J Immunol. 1995; 154(1):17-25. (Biology). 查看参考
Bowman MR, Crimmins MA, Yetz-Aldape J, Kriz R, Kelleher K, Herrmann S. The cloning of CD70 and its identification as the ligand for CD27. J Immunol. 1994; 152(4):1756-1761. (Biology). 查看参考
Camerini D, Walz G, Loenen WA, Borst J, Seed B. The T cell activation antigen CD27 is a member of the nerve growth factor/tumor necrosis factor receptor gene family. J Immunol. 1991; 147(9):3165-3169. (Biology). 查看参考
Centers for Disease Control. Update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in healthcare settings. MMWR. 1988; 37:377-388. (Biology).
Clinical and Laboratory Standards Institute. 2005. (Biology).
De Jong R, Brouwer M, Hooibrink B, Van der Pouw-Kraan T, Miedema F, Van Lier RA. The CD27- subset of peripheral blood memory CD4+ lymphocytes contains functionally differentiated T lymphocytes that develop by persistent antigenic stimulation in vivo. Eur J Immunol. 1992; 22(4):993-999. (Biology). 查看参考
Hintzen RQ, De Jong R, Lens SM, Brouwer M, Baars P, Van Lier RA. Regulation of CD27 expression on subsets of mature T lymphocytes. J Immunol. 1993; 151:2426-2435. (Biology).
Hintzen RQ, Lens SM, Beckmann MP, Goodwin RG, Lynch D, van Lier RA. Characterization of the human CD27 ligand, a novel member of the TNF gene family. J Immunol. 1994; 152(4):1762-1773. (Biology). 查看参考
Hintzen RQ, de Jong R, Hack CE, et al. A soluble form of the human T cell differentiation antigen CD27 is released after triggering of the TCR/CD3 complex. J Immunol. 1991; 147(1):29-35. (Biology). 查看参考
Kobata T, Agematsu K, Kameoka J, Schlossman SF, Morimoto C. CD27 is a signal-transducing molecule involved in CD45RA+ naive T cell costimulation. J Immunol. 1994; 153(12):5422-5432. (Biology). 查看参考
Maurer D, Fischer GF, Fae I, et al. IgM and IgG but not cytokine secretion is restricted to the CD27+ B lymphocyte subset. J Immunol. 1992; 148(12):3700-3705. (Biology). 查看参考
Morimoto C. Schlossman SF, Boumsell L, Gilks W, et al, ed. Leucocyte Typing V: White Cell Differentiation Antigens. 1995:356-357.
Reiter C. T9. Cluster report: CD27. In: Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:350.

查看所有文件 (13)

655157 Rev. 1

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