CD16 PE-Cy™7

BD™ CD16 PE-Cy™7

Name: CD16 PE-Cy™7
Brand: BD™
SKU: 335806

克隆 B73.1

(ASR)

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科学资源

商品详情

Brand: BD™

Alternative Name: IgG Fc receptor III; IGFR3; FCG3; FCGR3; FCGRIII; FcγRIII

Reactivity: Human

Isotype: Mouse BALB/c IgG1, κ

Immunogen: Human NK Cells

Application: Flow cytometry

Concentration: 100 μg/mL

Vol. Per Test: 5 μL

Workshop Number: IV NL402

Entrez Gene ID: 916

Storage Buffer: Phosphate buffered saline with gelatin and 0.1% sodium azide.

Regulatory Status: ASR

准备和存储

Store vials at 2° to 8°C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.

335806 Rev. 1

抗体详情

B73.1

CD16 (Leu-11a), clone NKP15, is derived from hybridization of mouse P3-X63-Ag8 myeloma cells with spleen cells from BALB/c mice immunized with large granular lymphocytes.

CD16 (Leu-11b), clone GO22, is derived from hybridization of mouse P3-X63 myeloma cells with spleen cells from BALB/c mice immunized with granulocytes.

CD16 (Leu-11c), clone B73.1, is derived from hybridization of mouse P3-X63-Ag8.653 myeloma cells with spleen cells from BALB/c mice immunized with NK lymphocytes.

CD16 antibodies recognize an Mr 50- to 65-kilodalton (kd) antigen present on human natural killer (NK) lymphocytes that is the IgG Fc receptor III.

335806 Rev. 1

格式详情

PE-Cy7

PE-Cy7 dye is a part of the BD PE family of dyes. This tandem fluorochrome is comprised of a R-Phycoerythrin (PE) donor that has excitation maxima (Ex Max) of 496-nm and 566-nm and an acceptor dye, Cy™7, with an emission maximum (Em Max) at 781-nm. PE can be excited by the Blue (488-nm), Green (532-nm) and yellow-green (561-nm) lasers and detected using an optical filter centered near 781 nm (e.g., a 760/60-nm bandpass filter). The donor dye can be excited by the Blue (488-nm), Green (532-nm) and yellow-green (561-nm) lasers and the acceptor dye can be excited by the Red (627–640-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

格式: PE-Cy7

激发源: Yellow-Green 488 nm, 532 nm, 561 nm

最多激发源: 496 nm, 566 nm

最多发射源: 781 nm

335806 Rev.1

报价单和参考

研发参考 (10)

Gerosa F, Baldani-Guerra B, Nisii C, Marchesini V, Carra G, Trinchieri G. Reciprocal activating interaction between natural killer cells and dendritic cells. J Exp Med. 2002; 195(3):327-333. (Biology). 查看参考
Lanier LL, Kipps TJ, Phillips JH. Functional properties of a unique subset of cytotoxic CD3+ T lymphocytes that express Fc receptors for IgG (CD16/Leu-11 antigen). J Exp Med. 1985; 162(6):2089-2106. (Biology). 查看参考
Lanier LL, Le AM, Civin CI, Loken MR, Phillips JH. The relationship of CD16 (Leu-11) and Leu-19 (NKH-1) antigen expression on human peripheral blood NK cells and cytotoxic T lymphocytes. J Immunol. 1986; 136(12):4480-4486. (Biology). 查看参考
Lanier LL, Le AM, Phillips JH, Warner NL, Babcock GF. Subpopulations of human natural killer cells defined by expression of the Leu-7 (HNK-1) and Leu-11 (NK-15) antigens. J Immunol. 1983; 131(4):1789-1796. (Biology). 查看参考
Perussia B, Acuto O, Terhorst C, et al. Human natural killer cells analyzed by B73-1, a monoclonal antibody blocking Fc receptor functions, II: studies of B73-1 antibody-antigen interaction on the lymphocyte membrane. J Immunol. 1983; 130:2142-2148. (Biology).
Perussia B, Starr S, Abraham S, Fanning V, Trinchieri G. Human natural killer cells analyzed by B73.1, a monoclonal antibody blocking Fc receptor functions. I. Characterization of the lymphocyte subset reactive with B73.1. J Immunol. 1983; 130(5):2133-2141. (Biology). 查看参考
Perussia B, Trinchieri G, Jackson A, et al. The Fc receptor for IgG on human natural killer cells: phenotypic, functional, and comparative studies with monoclonal antibodies. J Immunol. 1984; 133(1):180-189. (Biology). 查看参考
Phillips JH, Babcock GF. A monoclonal antibody reactive against purified human natural killer cells and granuocytes. Immunol Letters. 1983; 6:143. (Biology).
Schmidt RE. Non-lineage/natural killer section report: new and previously defined clusters. In: Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:517-542.
Thompson J, Goeken N, Brown S, Rhodes J. Phenotypic definition of human monocyte/macrophage subpopulations by monoclonal antibodies, II: Distinction of non-T, non-B natural killer cells (NK) from antigen-presenting stimulating cells in the mixed lymphocyte response (MLR) Presented at the American Association for Clinical Histocompatibility Testing. (Biology).

查看所有文件 (10)

335806 Rev. 1

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