Skip to main content Skip to navigation
Regulatory T-Cell Function Kit

BD FastImmune™ Regulatory T-Cell Function Kit

Down Arrow Up Arrow

BD FastImmune™
Flow cytometry


Naturally occurring human CD4+ regulatory T cells (Tregs) are critical mediators of self-tolerance and suppress aberrant or inappropriate immune responses that can lead to conditions such as type 1 diabetes, rheumatoid arthritis, and graft-vs-host disease. The BD FastImmune™ kit measures expression of the activation markers CD154 and/or CD69 on effector T cells. Reduced expression of these markers in the presence of Tregs can indicate Treg suppressive capacity. The assay is performed in 96-well microplates using peripheral blood mononuclear cells (PBMCs) activated in the presence and absence of Tregs. It is a short-term assay that can be set up and analyzed in approximately one day. The T-cell markers, CD3 and CD4, are included in the kit for T-cell gating purposes, and CD25 is included for defining the Treg population.

Contents: Regulatory T-Cell function cocktail (CD3 PerCP-Cy™5.5, CD4 FITC, CD25 PE)| CD69 PE-Cy™7| CD154 APC| EDTA.


Store reagents at 2°C to 8°C. Conjugated forms should not be frozen and should be protected from exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.

648956 Rev. 1
Down Arrow Up Arrow

研发参考 (8)

  1. Ashokkumar C, Talukdar A, Sun Q, et al. Allospecific CD154+ T cells associate with rejection risk after pediatric liver transplantation.. Am J Transplant. 2009; 9(1):179-91. (Biology). 查看参考
  2. Baecher-Allan C, Brown JA, Freeman GJ, Hafler DA. CD4+CD25high regulatory cells in human peripheral blood. J Immunol. 2001; 167:1245-1253. (Biology).
  3. Bour-Jordan H, Bluestone JA. Regulating the regulators: costimulatory signals control the homeostasis and function of regulatory T cells.. Immunol Rev. 2009; 229(1):41-66. (Biology). 查看参考
  4. Centers for Disease Control. Update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in healthcare settings. MMWR. 1988; 37:377-388. (Biology).
  5. Chattopadhyay PK, Yu J, Roederer M. A live-cell assay to detect antigen-specific CD4+ T cells with diverse cytokine profiles.. Nat Med. 2005; 11(10):1113-7. (Biology). 查看参考
  6. Clinical and Laboratory Standards Institute. 2005. (Biology).
  7. Disis ML, dela Rosa C, Goodell V, et al. Maximizing the retention of antigen specific lymphocyte function after cryopreservation. J Immunol Methods. 2006; 308:13-18. (Biology).
  8. Frentsch M, Arbach O, Kirchhoff D, et al. Direct access to CD4+ T cells specific for defined antigens according to CD154 expression.. Nat Med. 2005; 11(10):1118-24. (Biology). 查看参考
查看所有文件 (8) 查看更少内容
648956 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.