描述
The BD Leucocount™ kit consists of BD Leucocount™ reagent (propidium iodide fluorescent dye) and BD Trucount™ tubes and is intended for use with the BD FACSLyric™, BD FACSCalibur™, BD FACSort™, BD FACScan™, and BD FACSVia™ flow cytometer systems, or a flow cytometer equipped with a 488-nm laser able to threshold on propidium iodide fluorescence, for enumerating residual white blood cells (rWBCs) in leucoreduced blood products.
准备和存储
• Store BD Leucocount reagent at 2°C– 8°C. Do not use after the expiration date shown on the label. • Avoid unnecessary exposure of the reagent to light. • Do not freeze the reagents or expose them to direct light during storage or incubation with cells. Keep the reagent vials dry. • Store BD Trucount tubes in their original foil pouch at 2°C–25°C. To avoid potential condensation, open the pouch only after it has reached room temperature and carefully reseal the pouch immediately after removing a tube. Examine the desiccant each time you open the pouch. If the desiccant has turned from blue to lavender, discard the remaining tubes. Use tubes within 1 hour after removal from the foil pouch. Once the pouch has been opened, the tubes are stable for 1 month. Do not use beyond the expiration date indicated on the packaging.
| 描述 | 数量/尺寸 | 零件号 | EntrezGene ID |
|---|---|---|---|
| Leucocount Reagent | 50 Tests (1 ea) | 91-1211 | N/A |
| BD Trucount tubes (25 tubes) | 1 Each (2 ea) | 91-0786 | N/A |
研发参考 (11)
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Bull BS, Breton-Corius J. Beutler E, Lichtman MA, Coller BS, Kripps TJ, ed. Williams Hematology. New York, NY: McGraw-Hill, Inc ; 1995:349.
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Center for Biologics Evaluation and Research. Recommendations and Licensure Requirements for Leukocyte-Reduced Blood Products. 1996. (Biology).
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Centers for Disease Control. Update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in healthcare settings. MMWR. 1988; 37:377-388. (Biology).
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Clinical and Laboratory Standards Institute. 2005. (Biology).
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Dumont LJ, Dzik WH, Rebulla P, Brandwein H Practical guidelines for process validation and process control of white cell-reduced blood components: report of the Biomedical Excellence for Safer Transfusion (BEST) Working Party of the International Society of Blood Transfusion (ISBT). Transfusion. 1996; 11-20. (Biology).
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Rebulla P, Porretti L, Bertolini F, et al. White cellreduced red cells prepared by filtration: a critical evaluation of current filters and methods for counting residual white blood cells. Transfusion. 1993; 33:128-133. (Biology).
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Sniecinski I, O'Donnell MR, Nowicki B, Hill LR. Prevention of refractoriness and HLAalloimmunization using filtered blood products. Blood. 1988; 71:1402-1407. (Biology).
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VanCott, J, H Staats, et al. Regulation of mucosal and systematic antibody responses by T helper cell subsets, macrophages, and derived cytokines following oral immunization with live recombinant Salmonella. J Immunol. 1996; 156:1504-1514. (Biology).
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Venglen-Tyler V, ed. Leukoreduction of RBC and platelet units. American Association of Blood Banks. 1996; 722-725. (Biology).
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Wenz B, Gurtlinger K, O'Toole A, Dugan E. Preparation of granulocyte-poor red blood cells by microaggregate filtration. A simplified method to minimize febrile transfusion reactions. Vox Sang. 1980; 39:282-287. (Biology).
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de Graan-Hentzen YCE, Gratama JW, Mudde GC, et al. Prevention of primary cytomegalovirus infection in patients with hematologic malignancies by intensive white cell depletion of blood products. Transfusion. 1989; 29:757-760. (Biology).
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