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Flow cytometric analysis using BD OptiBuild™ RY586 Hamster Anti-Mouse CD49a antibody (Cat. No. 753108; solid line histogram) on viable C1300 cells, with Autofluorescence Control (dotted line histogram). Flow cytometry was performed using a BD LSRFortessa™ X-20 Flow Cytometer System.
BD OptiBuild™ RY586 Hamster Anti-Rat/Mouse CD49a
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BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.
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- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Researchers should determine the optimal concentration of this reagent for their individual applications.
- The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
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The Ha31/8 monoclonal antibody specifically binds to the 180-kDa integrin α1 chain (CD49a), which is a transmembrane glycoprotein that non-covalently associates with the integrin β1 subunit (CD29) to form the α1β1 (complex known as VLA-1). VLA-1 has been reported to be expressed on activated T cells, monocytes, smooth muscle cells, and endothelial cells. It is a receptor for collagen and laminin. The Ha31/8 monoclonal antibody is specific for both rat and mouse CD49a. It has been reported that Ha31/8 antibody can block VLA-1-mediated binding of rat cells to collagen.
研发参考 (3)
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Hemler ME. VLA proteins in the integrin family: structures, functions, and their role on leukocytes. Annu Rev Immunol. 1990; 8:365-400. (Biology). 查看参考
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Mendrick DL, Kelly DM, duMont SS, Sandstrom DJ. Glomerular epithelial and mesangial cells differentially modulate the binding specificities of VLA-1 and VLA-2. Lab Invest. 1995; 72(3):367-375. (Immunogen: Immunohistochemistry). 查看参考
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Miyake S, Sakurai T, Okumura K, Yagita H. Identification of collagen and laminin receptor integrins on murine T lymphocytes. Eur J Immunol. 1994; 24(9):2000-2005. (Biology). 查看参考
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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
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