Old Browser
Looks like you're visiting us from {countryName}.
Would you like to stay on the current country site or be switched to your country?
监管状态图例
未经BD明确书面授权,严禁使用未经许可的任何商品。
准备和存储
推荐的实验流程
For immunohistochemical staining of cells expressing MHC class I antigen of the b haplotype, we recommend the use of the biotinylated anti-mouse H-2Kb mAb AF6-88.5 in our special formulation for immunohistochemistry, Cat. no. 550550.
商品通知
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
The 28-14-8 antibody reacts with the α3 domain of the H-2D[b] MHC class I alloantigen. The antibody binds to H-2D[b] in the presence or absence of the β2 microglobulin chain. It cross-reacts with the α3 domain of H-2L[d], but not with K[d] or D[d], and with H-2D[q] and/or L[q]. Reactivity with haplotypes k, f, p, r, and s has not been observed. mAb 28-14-8 has been shown to block H-2L[d]- specific and H- 2L[d]-restricted antigen-specific lysis of target cells by cytotoxic T lymphocytes (CTL), but it does not block recognition of H-2L[d] positive target cells by Ly-6G2-positive NK cells.
研发参考 (9)
-
Allen H, Fraser J, Flyer D, Calvin S, Flavell R. Beta 2-microglobulin is not required for cell surface expression of the murine class I histocompatibility antigen H-2Db or of a truncated H-2Db. Proc Natl Acad Sci U S A. 1986; 83(19):7447-7451. (Clone-specific: Immunoprecipitation). 查看参考
-
Allen H, Wraith D, Pala P, Askonas B, Flavell RA. Domain interactions of H-2 class I antigens alter cytotoxic T-cell recognition sites. Nature. 1984; 309(5965):279-281. (Clone-specific: Radioimmunoassay). 查看参考
-
Evans GA, Margulies DH, Shykind B, Seidman JG, Ozato K. Exon shuffling: mapping polymorphic determinants on hybrid mouse transplantation antigens. Nature. 1982; 300(5894):755-757. (Clone-specific: Radioimmunoassay). 查看参考
-
Kündig TM, Bachmann MF, DiPaolo C. Fibroblasts as efficient antigen-presenting cells in lymphoid organs. Science. 1995; 268(5215):1343-1347. (Clone-specific: Blocking). 查看参考
-
Mason LH, Ortaldo JR, Young HA, Kumar V, Bennett M, Anderson SK. Cloning and functional characteristics of murine large granular lymphocyte-1: a member of the Ly-49 gene family (Ly-49G2). J Exp Med. 1995; 182(2):293-303. (Clone-specific: Blocking). 查看参考
-
Orn A, Goodenow RS, Hood L. Product of a transferred H-2Ld gene acts as restriction element for LCMV-specific killer T cells. Nature. 1982; 297(5865):415. (Clone-specific: Blocking). 查看参考
-
Ozato K, Hansen TH, Sachs DH. Monoclonal antibodies to mouse MHC antigens. II. Antibodies to the H-2Ld antigen, the products of a third polymorphic locus of the mouse major histocompatibility complex. J Immunol. 1980; 125(6):2473-2477. (Immunogen: Cytotoxicity). 查看参考
-
Ozato K, Sachs DH. Monoclonal antibodies to mouse MHC antigens. III. Hybridoma antibodies reacting to antigens of the H-2b haplotype reveal genetic control of isotype expression. J Immunol. 1981; 126(1):317-321. (Immunogen: Cytotoxicity). 查看参考
-
Woodward JG, Orn A, Harmon RC, Goodenow RS, Hood L, Frelinger JA. Specific recognition of the product of a transferred major histocompatibility complex gene by cytotoxic T lymphocytes. Proc Natl Acad Sci U S A. 1982; 79(11):3613-3617. (Clone-specific: Blocking). 查看参考
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Report a Site Issue
This form is intended to help us improve our website experience. For other support, please visit our Contact Us page.