BD公司一直以来竭力提供优质产品，改进产品，以更好地为客户服务，帮助优化多色流式实验。为此，我们发起了一项旨在提高BD Horizon Brilliant™ Violet 605（BV605）研究专用（RUO）抗体试剂性能的倡议。BD Horizon™ BV605试剂将开始采用新的生产工艺，为您提供更好的光稳定性，使荧光亮度超越过去或者一致。此工艺变更会让您发现更多BV605 RUO的特异性。
BD Horizon™ BV605 RUO试剂（不包括BD OptiBuild™试剂）已经于2023年9月开始采用新工艺生产。预计需要18至24个月的时间才能在整个产品组合中实施此工艺变更。由于货号不变，试剂包装中将暂时包含产品通知，以表明只有特定货号发生了变更。
新工艺生产带来的变化：
- · 增强光稳定性
- · 相对于传统工艺能够提供更高或者同等的亮度
- · 货号、特异性、克隆或染料不变
- · 试剂包装中将暂时包含产品通知，以表明已发生变更
请参见下面的产品列表，详细了解哪些BD Horizon™ BV605 RUO试剂进行了变更。该表列出了采用传统工艺生产的最终批次和采用新工艺生产的第一批产品。
性能数据
通过比较旧版和新版BD Horizon™ BV605试剂，发现新版试剂的性能与旧版相似，甚至更好。
A. 使用BD Horizon™ BV605新工艺（蓝色直方图）或BD Horizon™ BV605传统工艺（橙色直方图）大鼠抗小鼠CD45试剂对小鼠脾细胞进行CD45染色的流式细胞术比较。
将来自Balb/c小鼠的脾脏白细胞与纯化的大鼠抗小鼠CD16/CD32抗体（小鼠BD Fc Block™试剂）一起预孵育。然后用0.25 µg/T的BV605大鼠抗小鼠CD45抗体对细胞进行染色。在分析前将BD Via-Probe™细胞活性7-AAD溶液立即加入细胞中。显示CD45表达的荧光直方图源自具有活性（7-AAD-阴性）脾脏白细胞前向和侧向光散射特征的圈门事件。
B. 使用BD Horizon™ BV605新工艺（左图）或BD Horizon™ BV605传统工艺（右图）大鼠抗小鼠CD45试剂对小鼠脾细胞进行CD45染色的流式细胞术比较。
将来自Balb/c小鼠的脾脏白细胞与纯化的大鼠抗小鼠CD16/CD32抗体（小鼠BD Fc Block™试剂）一起预孵育。然后用0.25 µg/T的BV605大鼠抗小鼠CD45抗体对细胞进行染色。在分析前将BD Via-Probe™细胞活性7-AAD溶液立即加入细胞中。显示CD45与侧向光散射（SSC-A）信号相关表达的双变量伪色密度图源自具有活性（7-AAD-阴性）脾脏白细胞前向和侧向光散射特征的圈门事件。
A. 使用BD Horizon™ BV605新工艺（蓝色直方图）或BD Horizon™ BV605传统工艺（橙色直方图）仓鼠抗小鼠CD3e试剂对小鼠脾细胞进行CD3e染色的流式细胞术比较。
将来自Balb/c小鼠的脾脏白细胞与纯化的大鼠抗小鼠CD16/CD32抗体（小鼠BD Fc Block™试剂）一起预孵育。然后用0.5 µg/T的BV605仓鼠抗小鼠CD3e抗体对细胞进行染色。在分析前将BD Via-Probe™细胞活性7-AAD溶液立即加入细胞中。显示CD3e表达的荧光直方图源自具有活性（7-AAD-阴性）脾脏白细胞前向和侧向光散射特征的圈门事件。
B. 使用BD Horizon™ BV605新工艺（左图）或BD Horizon™ BV605传统工艺（右图）仓鼠抗小鼠CD3e试剂对小鼠脾细胞进行CD3e染色的双色流式细胞术比较。
将来自Balb/c小鼠的脾脏白细胞与纯化的大鼠抗小鼠CD16/CD32抗体（小鼠BD Fc Block™试剂）一起预孵育。然后用0.5 µg/T的FITC大鼠抗小鼠CD4、FITC大鼠抗小鼠CD8a抗体以及BV605仓鼠抗小鼠CD3e抗体对细胞进行染色。在分析前将BD Via-Probe™细胞活性7-AAD溶液立即加入细胞中。双变量伪色密度图显示了CD3e与CD4和CD8a相关表达模式，用于具有活性（7-AAD-阴性）脾脏白细胞前向和侧向光散射特征的圈门事件。
A. 使用BD Horizon™ BV605新工艺（蓝色直方图）或BD Horizon™ BV605传统工艺（橙色直方图）小鼠抗人CD19试剂对人外周血淋巴细胞进行CD19染色的流式细胞术比较。
用0.25 µg/T的BV605小鼠抗人CD19抗体对全血进行染色。用BD FACS™溶血素对红细胞进行裂解。显示CD19表达的荧光直方图源自具有完整淋巴细胞前向和侧向光散射特征的圈门事件。
B. 使用BD Horizon™ BV605新工艺（左图）或BD Horizon™ BV605传统工艺（右图）小鼠抗人CD19抗体进行CD19染色的流式细胞术比较。
用0.25 µg/T的BV605小鼠抗人CD19抗体对全血进行染色。用BD FACS™溶血素对红细胞进行裂解。显示CD19与侧向光散射（SSC-A）信号相关表达的双变量伪色密度图源自具有完整白细胞前向和侧向光散射特征的圈门事件。
Product Transition Chart
The list below identifies the last lot using legacy process and the first lot incorporating the new manufacturing process for each catalog number affected. This chart will be updated as new catalog numbers are added to the program.
| BV605 RUO Reagent Cat. No. | Species | Description | Clone | Last Lot Legacy Process | First Lot New Process |
|---|---|---|---|---|---|
| 562652 | Iso Ctrl | Ms IgG1 Kappa ItCl BV605 X40 50µg | X40 | 3152703 | 4094046 |
| 562653 | Human | Hu CD19 BV605 SJ25C1 100Tst | SJ25C1 | 3184309 | 3303972 |
| 562654 | Human | Hu CD19 BV605 SJ25C1 25 Tests | SJ25C1 | 3184316 | 3325088 |
| 562655 | Human | Hu CD27 BV605 L128 100 Tests | L128 | 3184317 | 3321714 |
| 562656 | Human/Mouse | Hu CD27 BV605 L128 25 Tests | L128 | 3184318 | 4071598 |
| 562658 | Human | Hu CD4 BV605 RPA-T4 100 Tests | RPA-T4 | 3082471 | 4012899 |
| 562659 | Human | Hu CD4 BV605 RPA-T4 25 Tests | RPA-T4 | 3184307 | 3352996 |
| 562660 | Human | Hu CD25 BV605 2A3 100 Tests | 2A3 | 3061707 | 4102081 |
| 562661 | Human | Hu CD25 BV605 2A3 25 Tests | 2A3 | 2188457 | 4009425 |
| 562662 | Human | Hu CD127 BV605 HIL-7R-M21 50 Tests | HIL-7R-M21 | 3226376 | 3321642 |
| 562665 | Human | Hu CD38 BV605 HB7 100 Tests | HB7 | 1322172 | 3352989 |
| 562687 | Human | Hu CD117 BV605 104D2 50 Tests | 104D2 | 3130919 | 4030215 |
| 562719 | Human | Hu CD62L BV605 DREG-56 100Tst | DREG-56 | 3180063 | 4064570 |
| 562721 | Human | Hu CD11B BV605 ICRF44 100Tst | ICRF44 | 3233364 | 3272397 |
| 562723 | Human | Hu CD11B BV605 ICRF44 25Tst | ICRF44 | 3146211 | 3272395 |
| 562724 | Human | Hu CD196 CCR6 BV605 11A9 50Tst | 11A9 | 3177669 | 3264373 |
| 562778 | Iso Ctrl | Ms IgG2A KPA ITCL BV605 G155-178 50ug | G155-178 | 3187151 | 4008573 |
| 562779 | Human | Hu CD56 BV605 NCAM16.2 25Tst | NCAM16.2 | 3156323 | 3317058 |
| 562780 | Human | Hu CD56 BV605 NCAM16.2 100Tst | NCAM16.2 | 3156321 | 3317055 |
| 562788 | Human | Hu CD24 BV605 ML5 100Tst | ML5 | 3122666 | 3321650 |
| 562790 | Human | Hu CD45RO BV605 UCHL1 25Tst | UCHL1 | 3146225 | 4015615 |
| 562791 | Human | Hu CD45RO BV605 UCHL1 100Tst | UCHL1 | 3214605 | 4015613 |
| 562840 | Mouse | Ms TCR Beta Chain BV605 H57-597 50ug | H57-597 | 3187152 | 3317261 |
| 562843 | Hu/NHP | Hu/NHP CD4 BV605 L200 50Tst | L200 | 3163083 | 4038780 |
| 562844 | Human | Hu HLA-DR BV605 G46-6 25Tst | G46-6 | 3195112 | 3306279 |
| 562845 | Human | Hu HLA-DR BV605 G46-6 100Tst | G46-6 | 3177647 | 3355528 |
| 562853 | Human | Hu CD200 BV605 MRC OX-104 50Tst | OX-104 | 3177627 | 3304433 |
| 562855 | Human | Hu CD31 BV605 WM59 50Tst | WM59 | 3187153 | 4008617 |
| 562886 | Human | Hu CD45RA BV605 HI100 50Tst | HI100 | 3184308 | 3325094 |
| 562906 | Human | Hu CD194 BV605 1G1 50Tst | 1G1 | 3201639 | 4015670 |
| 562974 | Human | Hu IFN-GMA BV605 B27 50Tst | B27 | 3184319 | 4003676 |
| 562976 | Human | Hu CD28 BV605 CD28.2 100Tst | CD28.2 | 3187154 | 3318354 |
| 562977 | Human | Hu IgM BV605 G20-127 50Tst | G20-127 | 2286958 | 3352989 |
| 562978 | Human | Hu CD10 BV605 HI10A 100Tst | HI10A | 3038321 | 3318361 |
| 562979 | Human | Hu CD15 BV605 W6D3 25Tst | W6D3 | 3107761 | 3258335 |
| 562980 | Human | Hu CD15 BV605 W6D3 100Tst | W6D3 | 3107749 | 3258341 |
| 562987 | Iso Ctrl | Rat IgG1 Lam ITCL BV605 A110-1 50ug | A110-1 | 2293021 | 4025405 |
| 562989 | Human | Hu CD69 BV605 FN50 100Tst | FN50 | 3130907 | 3282053 |
| 562993 | Iso Ctrl | Rat IgG1 Kpa ITCL BV605 R3-34 50ug | R3-34 | 3054643 | 4051082 |
| 562994 | Hu/NHP | Hu/NHP CD3 BV605 SP34-2 50Tst | SP34-2 | 3214607 | 3352993 |
| 562999 | Human | Hu CD86 BV605 2331(FUN-1) 100Tst | 2331(FUN-1) | 2340591 | 3306989 |
| 563003 | Mouse | Ms IgD BV605 11-26C.2A 50ug | 11-26C.2A | 3180043 | 4127734 |
| 563004 | Mouse | Ms CD3e BV605 145-2C11 50ug | 145-2C11 | 3180100 | 3303974 |
| 563005 | Mouse | Ms LY-6G BV605 1A8 50ug | 1A8 | 3187156 | 3289454 |
| 563006 | Mouse | Ms CD16/CD32 BV605 2.4G2 50ug | 2.4G2 | 3187157 | 3325100 |
| 563008 | Mouse | Ms CD90.2 BV605 53-2.1 50ug | 53-2.1 | 3226389 | 4003693 |
| 563009 | Iso Ctrl | Ham IgG1 Kpa Itcl BV605 A19-3 50ug | A19-3 | 3214604 | 4103864 |
| 563012 | Iso Ctrl | Ham IgG2 Kpa Itcl BV605 B81-3 50ug | B81-3 | 3130755 | 3272224 |
| 563013 | Mouse | Ms CD71 BV605 C2 50ug | C2 | 3172612 | 4051083 |
| 563015 | Hu/Ms | CD11B BV605 M1/70 50ug | M1/70 | 3156259 | 3326985 |
| 563051 | Mouse | Ms CD45.2 BV605 104 50ug | 104 | 3205318 | 3317094 |
| 563052 | Mouse | Ms CD80 BV605 16-10A1 50ug | 16-10A1 | 3130908 | 3289439 |
| 563053 | Mouse | Ms CD45 BV605 30-F11 50ug | 30-F11 | 3187158 | 3289433 |
| 563054 | Iso Ctrl | Ham IgG1 Lam Itcl BV605 G235-2356 50ug | G235-2356 | 3124240 | 4102598 |
| 563057 | Mouse | Ms CD11c BV605 HL3 50ug | HL3 | 3180434 | 3291610 |
| 563058 | Mouse | Ms CD44 BV605 IM7 50ug | IM7 | 3226388 | 3347950 |
| 563059 | Mouse | Ms CD279 (PD-1) BV605 J43 50ug | J43 | 3184010 | 3236998 |
| 563060 | Mouse | Ms CD24 BV605 M1/69 50ug | M1/69 | 3109644 | 4025066 |
| 563061 | Mouse | Ms CD25 BV605 PC61 50ug | PC61 | 3170078 | 3324561 |
| 563099 | Iso Ctrl | Ms IgG2b Kpa Itcl BV605 MPC-11 50ug | MPC-11 | 3180455 | 4015986 |
| 563119 | Human/Mouse | SSEA-4 BV605 MC813-70 50Tst | MC813-70 | 3226411 | 4064379 |
| 563144 | Iso Ctrl | Rat IgG2a Kpa ItCl BV605 R35-95 50ug | R35-95 | 3033368 | 3268421 |
| 563147 | Mouse | Ms CD138 BV605 281-2 50ug | 281-2 | 3101304 | 4051085 |
| 563148 | Mouse | Ms CD19 BV605 1D3 50ug | 1D3 | 3130912 | 3303940 |
| 563151 | Mouse | Ms CD4 BV605 RM4-5 50ug | RM4-5 | 3187160 | 3272245 |
| 563152 | Mouse | Ms CD8a BV605 53-6.7 50ug | 53-6.7 | 3184323 | 3314867 |
| 563172 | Human | Hu CD16 BV605 3G8 100Tst | 3G8 | 3165796 | 3304209 |
| 563173 | Human | Hu CD16 BV605 3G8 25Tst | 3G8 | 3165798 | 3300504 |
| 563182 | Human | Hu CD326 BV605 EBA-1 50Tst | EBA-1 | 3187161 | 4032497 |
| 563199 | Human | Hu CD73 BV605 AD2 100Tst | AD2 | 3184333 | 3352962 |
| 563217 | Human | Hu CD3 BV605 SK7 25Tst | SK7 | 3188262 | 3325110 |
| 563219 | Human | Hu CD3 BV605 SK7 100Tst | SK7 | 3188260 | 3333038 |
| 563220 | Mouse | Ms NK-1.1 BV605 PK136 50ug | PK136 | 3072706 | 3300378 |
| 563245 | Human | Hu CD279 (PD-1) BV605 EH12.1 50Tst | EH12.1 | 3229830 | 3317240 |
| 563246 | Human | Hu IgG BV605 G18-145 50Tst | G18-145 | 3101307 | 3346936 |
| 563252 | Mouse | Ms CD62L BV605 MEL-14 50ug | MEL-14 | 3221656 | 3318364 |
| 563274 | Mouse | Ms IgG3 Kpa ItCl BV605 J606 50ug | J606 | 3089780 | 4051096 |
| 563285 | Mouse | Ms IgG1 BV605 A85-1 50ug | A85-1 | 3061444 | 3325996 |
| 563290 | Mouse | Ms CD69 BV605 H1.2F3 50ug | H1.2F3 | 3172620 | 3317269 |
| 563294 | Human | Hu CD138 BV605 MI15 50Tst | MI15 | 3184339 | 3338130 |
| 563299 | Mouse | Ms Ly-6G Ly-6C BV605 RB6-8C5 50ug | RB6-8C5 | 2355968 | 3303937 |
| 563313 | Human | Hu IgD BV605 IA6-2 50Tst | IA6-2 | 3214603 | 3289379 |
| 563315 | Human | Hu CD80 BV605 L307.4 100Tst | L307.4 | 2004399 | 3317122 |
| 563341 | Human | Hu TSLP Rcptr BV605 1F11/TSLPR 50Tst | 1F11/TSLPR | 2311550 | 3318336 |
| 563365 | Mouse | Ms CD27 BV605 LG.3A10 50ug | LG.3A10 | 3221655 | 4025222 |
| 563378 | Human | Hu CD43 BV605 1G10 100Tst | 1G10 | 3117875 | 3320498 |
| 563379 | Human | Hu CD195 BV605 2D7/CCR5 50Tst | 2D7/CCR5 | 3187162 | 3318351 |
| 563384 | Human | Hu CD337 (NKp30) BV605 p30-15 100Tst | p30-15 | 3180184 | 4015673 |
| 563413 | Mouse | Ms I-A I-E BV605 M5/114.15.2 50ug | M5/114 | 3023064 | 3331473 |
| 563708 | Mouse | Ms CD45R/B220 BV605 RA3-6B2 50ug | RA3-6B2 | 3214606 | 4031707 |
| 563711 | Human | Hu CCR7 (CD197) BV605 3D12 50Tst | 3D12 | 3124042 | 3325026 |
| 563759 | Human | Hu CD47 BV605 B6H12 100Tst | B6H12 | 3061620 | 4120146 |
| 563911 | Mouse | Ms IL-2 BV605 JES6-5H4 50ug | JES6-5H4 | 1124862 | 3303936 |
| 563929 | Human | Hu CD11c BV605 B-ly6 100Tst | B-ly6 | 3191603 | 3250877 |
| 563930 | Human | Hu CD11c BV605 B-ly6 25Tst | B-ly6 | 3151670 | 3250871 |
| 563945 | Human | Hu CD5 BV605 UCHT2 100Tst | UCHT2 | 3207996 | 4015978 |
| 563947 | Human | Hu IL-2 BV605 5344.111 50Tst | 5344.111 | 2329180 | 4015989 |
| 564007 | Mouse | Ms IL-4 BV605 11B11 50ug | 11B11 | 3184338 | 3289362 |
| 564009 | Mouse | Ms CD3 MolCpx BV605 17A2 50ug | 17A2 | 3129123 | 4053283 |
| 564013 | Mouse | Ms KLRG1 BV605 2F1 50ug | 2F1 | 3184341 | 4037172 |
| 564024 | Mouse | Ms IgG2a BV605 R19-15 50ug | R19-15 | 3156261 | 3318343 |
| 564032 | Human | Hu CD183 BV605 1C6/CXCR3 50Tst | 1C6/CXCR3 | 3027125 | 4024020 |
| 564047 | Human | Hu CD45 BV605 HI30 100Tst | HI30 | 3214600 | 4023509 |
| 564048 | Human | Hu CD45 BV605 HI30 25Tst | HI30 | 3214602 | 4023508 |
| 564054 | Human | Hu CD14 BV605 M5E2 100Tst | M5E2 | 3186596 | 3317077 |
| 564055 | Human | Hu CD14 BV605 M5E2 25Tst | M5E2 | 3186599 | 3317082 |
| 564069 | Mouse | Ms CD335 (NKp46) BV605 29A1.4 50ug | 29A1.4 | 3122669 | 4074305 |
| 564082 | Mouse | Ms IL-10 BV605 JES5-16E3 50ug | JES5-16E3 | 3146243 | 4051100 |
| 564098 | NHP | NHP CD45 BV605 D058-1283 50Tst | D058-1283 | 3272260 | 3272260 |
| 564115 | Human | Hu CD8 BV605 SK1 25Tst | SK1 | 3226391 | 4003750 |
| 564116 | Human | Hu CD8 BV605 SK1 100Tst | SK1 | 3152701 | 3304605 |
| 564165 | Human | Hu IL-2 BV605 MQ1-17H12 100Tst | MQ1-17H12 | 3177373 | 3289377 |
| 564169 | Mouse | Ms IL-17A BV605 TC11-18H10 50ug | TC11-18H10 | 3186605 | 3325020 |
| 564188 | Human | Hu CD193 BV605 5E8 50Tst | 5.00E+08 | 3167223 | 4031716 |
| 564197 | Human | Hu CD123 BV605 7G3 50Tst | 7G3 | 3170136 | 3356249 |
| 564362 | Human | Hu CD58 BV605 1C3 100Tst | 1C3 | 3187771 | 4032500 |
| 564382 | Mouse | Ms NKG2A-C-E BV605 20d5 50ug | 20D5 | 3082252 | 4043893 |
| 564712 | Human | Hu CD3 BV605 HIT3a 100Tst | HIT3a | 3205274 | 3325022 |
| 566754 | Human | Hu CCR7 (CD197) BV605 2-L1-A 100Tst | 2-L1-A | 3184343 | 4019958 |
| 566755 | Human | Hu CCR7 (CD197) BV605 2-L1-A 25Tst | 2-L1-A | 3233350 | 4019957 |
| 566908 | Human | Hu CD4 BV605 SK3 100Tst | SK3 | 3206361 | 3341178 |
| 567122 | Human/Mouse | Ki-67 BV605 B56 50Tst | B56 | 3272815 | 3356336 |
| 567309 | Mouse | Ms CD150 BV605 Q38-480 50ug | Q38-480 | 3166636 | 4073249 |
| 567430 | Human | Hu HLA-E BV605 3D12/HLA-E 100Tst | 3D12/HLA-E | 3201026 | 3201026 |
| 567431 | Human | Hu HLA-E BV605 3D12/HLA-E 25Tst | 3D12/HLA-E | 3044505 | 3044505 |
| 567459 | Human | Ms CD45 BV605 I3/2.3 50ug | I3/2.3 | 2301267 | 3320503 |
| 567571 | Human | Hu CD25 BV605 BC96 100Tst | BC96 | 3201052 | 3285976 |
| 567572 | Human | Hu CD25 BV605 BC96 25Tst | BC96 | 2286776 | 3285974 |
| 567691 | Human | Hu CD39 BV605 A1/CD39 100Tst | A1/CD39 | 3104429 | 3264003 |
| 567692 | Human | Hu CD39 BV605 A1/CD39 25Tst | A1/CD39 | 3044518 | 3320515 |
| 567822 | Mouse | Ms CX3CR1 BV605 Z8-50 50ug | Z8-50 | 3080851 | 4046058 |
| 567962 | Human | Hu CD15 BV605 7C3.RMAB 100Tst | 7C3.RMAB | 3128425 | 3340832 |
| 567963 | Human | Hu CD15 BV605 7C3.RMAB 25Tst | 7C3.RMAB | 3128421 | 3335439 |
For Research Use Only. Not for use in diagnostic or therapeutic procedures.