BD Biosciences at CYTO 2025

Commercial Tutorials

Charting the course for the next 50 years of flow cytometry

Over the past 50 years, flow cytometry has evolved into a high-dimensional technique capable of providing deep spectral multiplexing at the single-cell level. The introduction of real-time imaging flow cytometry has further advanced the field, revealing how measurements of cell morphology and the spatial distribution of molecular analytes can provide biological insights with sub-cellular resolution.

In this tutorial, we’ll introduce you to the BD FACSDiscover^™ A8 Cell Analyzer, which combines high-parameter immunophenotyping through BD SpectralFX^™ Technology and real-time imaging flow cytometry through BD CellView^™ Image Technology. We'll demonstrate how these innovations enable unique applications and analytical possibilities, including the continuous expansion of measurable parameters, high-throughput data generation with a novel auto-sampling device, and seamless high-parameter panel portability between analyzers and sorters. Find out how this technology is charting the course for the next 50 years of flow cytometry, empowering rapid scientific discovery and advancing our understanding of cellular biology. | Watch the tutorial

Florian Mair, Managing Director of Flow Cytometry Facility and Immunologist, ETH Zurich

Bob Balderas, VP Biological Sciences, BD Biosciences

Peter Mage, Principal Engineer, Advanced Technologies, BD Biosciences

Aaron Middlebrook, Sr Staff Scientist, Biology, BD Biosciences

Facilitated by:

Eric Diebold, VP/GM, Instruments, Software & Informatics, BD Biosciences

Sunday, June 1

Time: 12:15 – 1:15 p.m.

Location: Mile High 2AB

Welcome to the next generation of cytometric data analysis: FlowJo™ Software Version 11

BD Biosciences is proud to unveil the brand-new FlowJo^™ Software version 11.0! Analyzing single-cell data using a comprehensive toolbox of manual and automated methods is crucial for completing any assay. Dr. John Quinn, Director of Science and Product Development at BD Bioinformatics, will present a live tutorial on the thoroughly redesigned release, its usage, and future development plans. The platform highlights include a streamlined sample hierarchy, a new Metadata Manager, built-in quality control, an overhauled Compensation/Unmixing Wizard, a Graph Gallery, virtual concatenation, built-in clustering and charts, simplified table and report creation, a built-in Image Viewer, and an immersive design. Additionally, leading scientists who participated in beta testing will share their insights on the key benefits of FlowJo^™ 11. | Watch the tutorial

John Quinn, Director, Science & Product Development, FlowJo Informatics, BD Biosciences

Facilitated by:

Eric Diebold, VP/GM, Instruments, Software & Informatics, BD Biosciences

Monday, June 2

Time: 12:15 – 1:15 p.m.

Location: Mile High 2AB

Missed the event? Register for an upcoming webinar. Register>

**PRODUCT UNVEILING**

Help us celebrate the monumental launch of the BD FACSDiscover^™ A8 Cell Analyzer, where state-of-the-art spectral flow cytometry and real-time imaging meet in one invaluable instrument. 

Time: 5:00 – 6:15 p.m.

Location: Booth #615

P095: Concurrent protein profiling of intracellular cytokines and phosphoproteins integrated into a high-parameter surface protein assay at the single-cell level | Download

Aruna Ayer, BD Biosciences

P103: Expanding our understanding of CITE-Seq to enable cost-saving and efficient multiomic single-cell experiments that consistently yield high-quality data | Download

Cynthia Sakofsky, BD Biosciences

P199: Enabling cross-instrument standardization using a dried down 20-color spectral and imaging immunophenotyping panel on the BD FACSDiscover^™ A8 Cell Analyzer 

Chip Lomas, BD Biosciences

P235: Loader performance and optimal setup for high-throughput data generation using BD FACSDiscover^™ A8 Cell Analyzer | Download

Rodrigo Pestana, BD Biosciences

P236: Marginal gains in small particle cytometry using BD FACSDiscover^™ S8 Cell Sorter and BD CellView^™ Image Technology providing improved precision & accuracy | Download

Aaron Tyznik, BD Biosciences

P269: Automated singlet discrimination in cytometry | Download

Mark Colasurdo, BD Biosciences

P289: Label free detection of circulating tumor cells using image derived features through a neural net classifier | Download

Aaron Middlebrook, BD Biosciences

P306: Mastering BD CellView^™ Image Technology: Optimizing imaging parameters for superior data generation and single cell sorting | Download

Lorena Hidalgo, BD Biosciences

P325: Deep learning-enabled cell phenotyping based on multi-channel label-free images from BD FACSDiscover^™ Platforms | Download

Keegan Owsley, BD Biosciences

Oral Presentation: Advancing cellular analysis: A comprehensive workflow using label-free spectral flow cytometry and imaging | Download

Laura Ferrer-Font, Scientific Solutions Manager, Medical & Scientific Affairs, BD Biosciences

Moen Sen, Senior Scientist, MSA Applied Research and Technology, BD Biosciences

Spectral Imaging Session 3

Time: 10:45 – 11:45 a.m.

Location: Mile High Ballroom 4D

P122: The Ultimate Predator: Tracking CD8+ cells using T-REX in a baboon model of HIV elite control | Download

Veronica Obregon-Perko, BD Biosciences

P270: Automated, multiparameter cell cycle analysis | Download

Mark Colasurdo, BD Biosciences

P286: From legends to labs: Unveiling IFNAR's role in obesity-related liver regeneration | Download

Ioannis Panetas, BD Biosciences

P298: Watch what you eat: Characterizing the phagocytotic microenvironment using machine learning on cytometric and imaging parameters | Download

John Quinn, BD Biosciences

P300: “What is essential is invisible to the eye”: Modernizing AML-MRD detection using unsupervised analysis | Download

Serena Di Cecilia, BD Biosciences

P304: Seeing the unseen: Label-free identification of cell apoptosis through deep learning | Download

Aaron Tyznik, BD Biosciences

Oral Presentation: Spectral hotspot analysis reveals unmixing-dependent spreading | Download

Peter Mage, Principal Engineer, Advanced Technologies, BD Biosciences

High Content/High Parameter Analysis

Time: 10:45  –  11:45 a.m.

Location: Mile High Ballroom 1D