BD FACSAria™ Cell Sorters

How is aseptic, high‑purity cell sorting achieved on BD FACSAria™ III and BD FACSAria^™ Fusion systems?

Aseptic, high‑purity sorting on BD FACSAria III or Fusion is achieved through several integrated system features, including:

• Integrated biosafety systems on the Fusion model, providing operator and sample protection.
• Precise fluidics with hydrodynamic focusing to maintain a stable, single‑cell stream.
• Highly sensitive cuvette‑based optical detection, which maximizes signal quality.
• Fixed‑alignment optics that ensure consistent, reproducible sorting performance.

How does a cuvette flow cell improve signal detection sensitivity in flow cytometry?

The cuvette flow cell improves sensitivity by:

• Providing fixed, stable optical alignment, reducing variability and maintaining optimal laser–sample interaction.
• Maximizing excitation and emission light capture through gel‑coupling to the objective lens.
• Enabling high signal‑to‑noise performance, even at high sorting speeds.
• Supporting advanced multicolor detection architectures, which enhances sensitivity for complex panels.

What types of biological assays can the BD FACSAria Cell Sorters support?

The BD FACSAria Cell Sorters are designed with broad flexibility, allowing them to accommodate a wide range of biological assays. This includes applications in immunology, cell biology, molecular biology, and multi‑parameter flow cytometry. Their adaptable configuration makes them suitable for both simple and complex experimental workflows.

Does the BD FACSAria Cell Sorter support cell viability workflows?

Yes. It supports cell viability–based assays by enabling gentle sorting conditions and providing options to maintain cell health throughout the process. This helps ensure high post-sort viability for sensitive or fragile cell populations.

What is BD FACS™ Accudrop Technology and how does it improve my workflow?

The BD FACS^™ Accudrop Technology is an automated setup and optimization feature that simplifies the droplet delay calibration required for accurate cell sorting.

It improves ease of use by:

• Automating a traditionally manual, time‑consuming step
• Increasing sorting accuracy
• Reducing user error
• Speeding up system setup, especially for new operators

How does the BD FACS^™ Accudrop Technology enhance automation?

The technology has automated calibration, which reduces the need for manual intervention and makes the sorting process faster and more consistent. This automation enhances overall workflow efficiency and helps ensure reproducibility across experiments and users.