CAR-T and Cell Therapy Research and Development

The BD Rhapsody^™ HT Single-Cell Analysis System enables reliable and reproducible high-throughput single-cell capture and minimal site-to-site variability making it suitable for translational, multi-center, multi-cohort studies. Multiomic studies on the BD Rhapsody^™ System analyzing both protein and mRNA (CITE-seq) reduce experimental time and provide complete, multiomic single-cell information. BD^® AbSeq Antibody-Oligonucleotide Conjugates allow high-parameter protein expression data (>100 cell surface and intracellular markers) alongside single-cell RNA-seq data to significantly improve your understanding of individual cells. Further, the next generation BD Rhapsody^™ TCR/BCR Next Multiomic Assays enable scientists to analyze the sequence of full-length TCR/BCR alongside single-cell protein and whole transcriptome mRNA analysis to comprehensively understand immune cell function. Using genomic and proteomic information from single-cell analysis, researchers can focus on cells of interest for research and a deeper understanding. We also provide a data analysis pipeline to support single-cell multiomics bioinformatic needs.

Flow cytometry is commonly used upstream of multiomics experiments and is also used as a tool for single-cell analysis and cell sorting for other downstream experiments. Key biological information and deeper insights can be obtained by isolating cells of interest with the BD FACSDiscover™ S8 Cell Sorter to gain further understanding of mechanisms of action. The BD FACSDiscover^™ S8 Cell Sorter with BD CellView^™ Image Technology and BD SpectralFX^™ Technology revolutionized the world of cell sorting as the first spectral sorter with sort-capable imaging. This means researchers can now see what they sort in real time based on morphological and spatial features or up to three fluorescence imaging parameters. One example is the ability to track whether specific transcription factors are localized in the cytoplasm or localized in the nucleus. These abilities could translate to more efficient workflows and accurate results from downstream analysis.

High-parameter flow cytometry experiments result in complex datasets. To analyze, visualize and organize data more effectively, especially when looking to define critical quality attributes, researchers are implementing machine learning to automate analysis. Using advanced computational tools within FlowJo™ Software, the leading software for flow cytometry analysis, you can unlock the insights from your data, with accessible features across a wide breadth of research applications. FlowJo™ Software enables flow cytometry data analysis with features for immunophenotyping, cell cycle, proliferation, kinetics studies, quantitative population comparison, plate screening assays and more. FlowJo™ Software contains a wide range of plugins to enable advanced analysis techniques and improve data workflows. Notably, the BD ElastiGate™ Technology, an automated gating algorithm available as a FlowJo™ Software plugin, has also been incorporated into the BD FACSuite™ Application using the identical algorithm. The use of the BD ElastiGate™ Technology on BD FACSLyric™ Systems combines the benefits of automated gating with the standardization and performance of the BD FACSLyric™ Flow Cytometry System.

In addition to managing the complex datasets being generated, there is another level of complexity around managing all the reagents, workflows and teams involved in generating that data. BD® Research Cloud is a unique cloud-based ecosystem for the organization of and collaboration around flow cytometry experiments. The powerful software allows you to track experiments through customizable workflows, manage your reagent inventory and titer information, design new panels and create BD FACSDiva™ Software-based experiments away from the cytometer enabling efficiency, visibility and collaboration for your discovery work.

Designing high complexity experiments requires access to a wide array of different reagents and technologies. Our portfolio of antibodies and fluorochromes, including BD Horizon Brilliant™, BD Horizon RealYellow™ and BD Horizon RealBlue™ Dyes, has more than 9,000 human immunology research reagents to support your cell therapy research. The family of BD Horizon RealYellow™ and BD Horizon RealBlue™ Reagents is bright, clean and laser specific. Driven by next-generation BD dye technology and AI-guided fluorochrome selection to optimize spectral positioning, these reagents are specially engineered to deliver reduced spillover and optimize resolution when used with other fluorochromes—helping to expand your experiments on both conventional and spectral flow cytometers.

Made on demand, BD OptiBuild™ Reagents provide lot-to-lot consistency and can be tailored to your requirements.

In addition to our broad portfolio of reagents, BD offers custom manufacturing services with any of our fluorochromes or antibody clones, as well as dye conjugation to your proprietary antibodies. Visit our Custom Reagents page to learn more about our custom manufacturing service. 

View the list of all the fluorochromes available at BD.