Cross-Reactive Antibodies for Human and Non Human Primate Research

Use a single flow cytometry panel across human and non-human primate (NHP) samples with cross-reactive antibody clones

Identify antibody clones that deliver consistent performance across human and NHP samples, enabling the use of a common flow cytometry panel across species. This resource is especially valuable for NHP toxicology, biodistribution, mechanistic, and translational studies, helping improve data comparability and continuity from preclinical research through clinical development.

We offer both ready-to-order NHP antibodies and custom solutions, including alternative fluorophore conjugations, higher‑concentration formats, and tailored formulations to help you build flow cytometry panels for both human and non-human primate samples.

If your research spans both human and NHP studies, the cross-reactivity chart below can help you identify antibody clones that recognize targets across species, enabling greater panel continuity and data comparability. The information is based on a combination of in-house testing and data from reputable external databases.

Please note that Waters Biosciences has not independently verified every cross-reactivity listed below.

Specificity	Clone Name	Human	Baboon	Cyno	Rhesus	Chimpanzee	African Green Monkey
CD2	RPA-2.10	+	+	+	+		R
CD3	SP34-2	+	+	+	+
CD4	L200	+	+	+	+
CD4	M-T477	+	+	+	+
CD4	RPA-T4	+	+	+	+
CD7	M-T701	+	+	+	+
CD8	OKT8	+	+	+	+
CD8	RPA-T8	+	+	+	+	+
CD8	SK1	+	+	+	+	+
CD8b	2ST8.5H7	+		+	+
CD10	HI10a	+	+	+	+		R
CD11a	HI111	+	+	+	+	R
CD11b	ICRF44	+	+	+	+		R
CD11c	3.9	+	R	R	+	+
CD13	WM15	R					R
CD14	M5E2	+	+	+	+	+	R
CD14	MφP9	+	+	+	+	+
CD16	3G8	+	+	+	+		R
CD18	6.7	+		+	+
CD19	HIB19	+			R
CD20	2H7	+	+	+	+		R
CD20	L27	+	+	+	+
CD21	B-ly4	+	+	+	+
CD25	2A3	+		+	+	+
CD25	BC96	+	R	R	R	+
CD25	M-A251	+	+	+	+	+
CD27	O323	R	R	R	R		R
CD27	L128	+	+	+	+
CD27	M-T271	+	+	+	+
CD28	CD28.2	+	+	+	+		R
CD28	L293	+	+	+	+
CD29	MAR4	+	+	+	+
CD31	WM59	+	+	+	+		R
CD32 (FcγRII)	FLI8.26	+	+	+	+
CD34	563	+		+	+	+
CD34	581	+				+
CD35	E11	R	R	R	R		R
CD39	A1	R	R	R	R		R
CD40	5C3	+	+	+	+
CD40	HB14	+	R	R	R		R
CD41a	HIP8	+	+	+	+		R
CD44v6	2F10	+			+
CD45RA	5H9	+	+	+	+
CD49d	9F10	+	+	+	+		R
CD49f	GoH3	R	R	R	R		R
CD51	23C6	R		R			R
CD56	R19-760	+	+	+	+
CD57	B159	+	+	+	+
CD58	MY31	+	+	+	+
CD59	NCAM16.2	+	+	+	+
CD59	p282 (H19)	+	+	+	+
CD61	VI-PL2	+	+	+	+		R
CD62L	SK11	+	+	+	+
CD62L	AC1.2	+	+	+	+
CD62L	AK-4	+		+	+
CD63	H5C6	+	+	+	+		R
CD64	10.1	R	R	R	R		R
CD69	FN50	+	+	+	+		R
CD73	AD2	R	R				R
CD74	LN2	R	R	R	R		R
CD79b	CB3-1	R	R	R	R		R
CD81	JS-81	+	+	+	+
CD81	1D6	+			+
CD83	HB15e	+	+	+	+		R
CD86	2331 (FUN-1)	+	+	+	+
CD86	IT2.2	+	R	R	R		R
CD89	A59	R	R	R	R		R
CD90	5E10	+	+	+	+
CD95 (Fas)	DX2	+	+	+	+	+	R
CD97	VIM3b	R			R
CD100	A8	R	R	R	R		R
CD107a	H4A3	R	R	R	R		R
CD119	GIR-208	R	R	R	R		R
CD152	BNI3	+	+	+	+
CD154 (CD40 Ligand)	24-31	+		R	R
CD154 (CD40 Ligand)	TRAP1	+	+	+	+
CD183 (CXCR3)	1C6/CXCR3	+	+	+	+
CD184 (CXCR4)	12G5	+	+	+	+		R
CD195 (CCR5)	3A9	+		+	+
CD196 (CCR6)	11A9	+	+	+	+
CD161	HP-3G10	R	R	R	R		R
CD162	KPL-1	R	R	R	R		R
CD163	GHI/61	R		R	R		R
CD166	3A6	R	R	R	R		R
CD243 (ABCB1)	UIC2	R	R				R
CD274 (PD-L1)	29E.2A3	R	R	R	R		R
CD278 (ICOS)	C398.4A	+	R	R	+		R
CD317	Y129	+	+	+	+
CD370	3A4/Clec9A	+		+
Annexin V	Annexin V	+		+	+	+
HLA-ABC	W6/32	R	R	R	R		R
HLA-ABC	G46-2.6	+	+	+	+
HLA-DR	G46-6	+	+	+	+
HLA-DR	L243	+	+	+	+		R
HLA-DR	TU36	+	+	+	+
HLA-DR, DP, DQ	Tu39	+	+	+	+
HU FOXP3	259D/C7	+	+	+	+
IFN-γ	4S.B3	+	+	+	+
IFN-γ	B27	+	+	+	+
IL-2	MQ1-17H12	+	+	+	+
IL-4	8D4-8	+		+	+
IL-4	MP4-25D2	+			+
IL-5	TRFK4	+	+	+	+
IL-5	TRFK5	+	+	+	+
IL-6	MQ2-6A3	+		+	+
Integrin β7	FIB504	R	R	R	R		R
Ki-67	B56	+	+	+	+
LOX-1	15C4	+	+	+	+
MCP-1	5D3-F7	+		+	+
MIP-1α	11A3	+		+	+
Rb	G3-245	+	+
Stat3	4/P-STAT3	+				+
Stat5 (pY694)	47/Stat5(pY694)	+	+		+
TCR-gamma/delta	B1	R	R	R	R		R
TNF	MAb1	+	+	+	+
TNF	MAb11	+	+	+	+
TRA-1-60 Antigen	TRA-1-60	+			+
TRA-1-81 Antigen	TRA-1-81	+			R

+ = positive - = negative R = reported (see reference below) blank = not tested/reported

Reference: Cell type-specific monoclonal antibody cross-reactivity screening in non-human primates and development of comparative immunophenotyping panels for CyTOF. Zachary B Bjornson-Hooper, Gabriela K Fragiadakis, Matthew H Spitzer, Deepthi Madhireddy, Kevin Hu, Kelly Lundsten, Garry P Nolan

When using cross‑reactive antibodies, you may observe differences in signal intensity or antigen density due to natural variation in expression profiles between species. To ensure accurate and reproducible results, we recommend a quick titration to determine the best staining concentration for each cell type and maximize resolution and specificity.

Representative flow cytometry data illustrating staining on human and rhesus whole blood for selected clones to highlight assay performance and species‑specific differences.

CD3 SP34-2

Rhesus

Multiparameter flow cytometric analysis of CD3 expression on Rhesus monkey peripheral blood leukocyte populations. Rhesus whole blood was stained with either BD Horizon™ RY743 Mouse IgG1, κ Isotype Control (Cat. No. 572209; Left Plot) or BD Horizon™ RY743 Mouse Anti-Human CD3 antibody (Cat. No. 572740/572800; Right Plot). The erythrocytes were lysed with BD FACS™ Lysing Solution (Cat. No. 349202). Flow cytometry and data analysis were performed using a BD FACSymphony™ A5 SE Cell Analyzer System and FlowJo™ Software.

Human

Multiparameter flow cytometric analysis of CD3 expression on Human peripheral blood leukocyte populations. Human whole blood was stained with either BD Horizon™ RY743 Mouse IgG1, κ Isotype Control (Cat. No. 572209; Left Plot) or BD Horizon™ RY743 Mouse Anti-Human CD3 antibody (Cat. No. 572740/572800; Right Plot) at the same dose as the Rhesus leukocytes. The erythrocytes were lysed with BD FACS Lysing Solution (Cat. No. 349202). Flow cytometry and data analysis were performed using a BD FACSymphony™ A5 SE Cell Analyzer System and FlowJo™ Software.

CD4 L200

Rhesus

Multiparameter flow cytometric analysis of CD4 expression on Rhesus macaque peripheral blood leukocyte populations. Rhesus macaque whole blood was stained with either BD Horizon™ RY775 Mouse IgG1, κ Isotype Control (Cat. No. 571408; Left Plot) or BD Horizon™ RY775 Mouse Anti-Human CD4 antibody (Cat. No. 571661/571739; Right Plot). The erythrocytes were lysed with BD FACS™ Lysing Solution (Cat. No. 349202). Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ Software.

Human

Multiparameter flow cytometric analysis of CD4 expression on Human peripheral blood leukocyte populations. Human whole blood was stained with either BD Horizon™ RY775 Mouse IgG1, κ Isotype Control (Cat. No. 571408; Left Plot) or BD Horizon™ RY775 Mouse Anti-Human CD4 antibody (Cat. No. 571661/571739; Right Plot). The erythrocytes were lysed with BD FACS™ Lysing Solution (Cat. No. 349202). Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ Software.

For additional information, you can access resources such as:

Clone comparison tool
Compare clones by specificity and see what formats we have.
NHP Reagent Resource
Find NHP antibodies and human-specific clones with reported cross-reactivity to support the same panel in human and NHP studies.

Explore Cross-reactive NHP Antibodies >

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