BD OMICS-Guard™ CRYO Preservation Buffer

Overview

Scaling single-cell studies across multiple sites, time points or patient cohorts requires sample storage solutions that keep pace with the demands of your research. The BD OMICS-Guard™ CRYO Preservation Buffer (PB) provides a simple, one-step long-term cryopreservation solution for single-cell suspensions, giving you the flexibility to collect, store and process samples on your schedule—without compromising sample biology.

Interested in short-term sample storage? Check out our BD OMICS-Guard™ Sample Preservation Buffer

BD OMICS Guard CRYO Sample Preservation Buffer

Long-Term Sample Storage

Store single-cell suspensions for months while preserving the biology on which your research depends

Distributed and Multi-Site Collection

Coordinate collection across sites and ship samples with confidence

Batch Processing

Consolidate sample processing across different collection timepoints to reduce variability and maximize operational efficiency

Longitudinal and Cohort Studies

Keep multi-timepoint and patient cohort studies on track with consistent, comparable sample conditions across collection windows

Features

Developed and optimized to address the need for high-quality biological sample preservation for long-term storage, the BD OMICS-Guard^™ CRYO PB provides:

One-step preservation
protocol with minimum hands-on time
Long-term sample storage
- Up to 5 months at –80 °C
- Up to 9 months in liquid nitrogen

Broad sample type compatibility
- Single-cell suspensions
- Whole blood
- Bulk tissue pieces
Available in two, easy-to-use formats: 50-mL bottle
or 12 x 1-mL vials

Compatibility across the BD Rhapsody™ System
assay portfolio

Interested in learning more? Talk to one of our specialists

Performance

PBMC samples stored in BD OMICS-Guard™ CRYO PB maintain high gene expression similar to fresh processed samples

Gene expression patterns are preserved in PBMC samples stored in BD OMICS-Guard™ CRYO PB at −80 °C over time. Gene expression levels (A) and sample and cell clustering patterns (B) were assessed in freshly processed samples and compared to samples stored for 10 and 60 days in BD OMICS-Guard™ CRYO PB. Gene detection comparisons across time display highly correlated expression levels with limited impact shown in sample and cell-type clustering data.

BD OMICS-Guard™ CRYO Preservation Buffer preserves representative sample cell type distributions across storage time points

Cell-type proportions are preserved in PBMC samples stored in BD OMICS-Guard™ CRYO PB at −80 °C over time. Cell-type composition was assessed in freshly processed samples and compared to samples stored for 10 and 60 days. Following sequencing and data analysis, stored samples showed less than 10% variation in cell-type proportions relative to fresh controls, demonstrating compositional stability across both timepoints.

Gene and protein expression performance are preserved after long-term (60-day) storage in BD OMICS-Guard™ CRYO PB

Gene and protein expression sensitivity are preserved in PBMC samples stored in BD OMICS-Guard™ CRYO PB at −80 °C. Gene expression (A) and protein expression (B) performance metrics were assessed in freshly processed samples and compared to samples stored in BD OMICS-Guard™ CRYO PB for 60 days. Following sequencing and data analysis, stored samples demonstrated equivalent sensitivity across both measures, indicating performance is maintained even after long-term storage.

Gene and Protein Expression Preservation

Direct whole blood sample storage is enabled in BD OMICS-Guard™ CRYO PB

Representative sample biology and assay performance are conserved in whole blood samples stored in cryopreservation conditions (in BD OMICS-Guard™ CRYO PB) for 3 months. Whole blood samples were either stored directly in BD OMICS-Guard™ CRYO PB or prepared into PBMCs and processed fresh. Following sequencing and data analysis, when stored sample’s metrics were compared to fresh processed samples, gene expression displayed a Pearson correlation value of 0.94 (A) and cell-type specific protein (B) and UMAP clustering patterns remained consistent.

Single-cell multomic ATAC-seq and TCR/BCR profiling data are preserved after long-term sample storage with BD OMICS-Guard™ CRYO PB

Data from samples processed after storage in BD OMICS-Guard™ CRYO PB were compared to aliquots of the same samples processed fresh in both the BD Rhapsody™ TCR/BCR Next and Multiomic ATAC-Seq Assays. Performance data for TCR/BCR pairing efficiency (A) and UMAP clustering overlays (B) illustrate no reduction in metrics or significant impact compared to fresh samples.