BD^® OMICS-Guard Sample Preservation Buffer

Protect transcriptome + proteome information for up to 72 hours

Overview

The BD^®OMICS-Guard Sample Preservation Buffer (SPB) is developed and optimized to address the need for high-quality biological sample preservation over an extended period of time when samples cannot be processed at the same time or location.

Simple one-step preservation protocol with minimum hands-on time
Preserves cells for a variety of downstream transcriptomic, epigenetic, proteomic and multiomic applications including RNA-seq, CITE-seq, TCR/BCR profiling, multiomic ATAC-seq,* flow cytometry and qPCR
Protects cell integrity and preserves different cell populations in your samples for up to 72 hours at 4 °C
Compatible across multiple sample types: PBMCs, bulk tissue samples and whole blood samples
Available in two, easy-to-use formats: 50-mL bottle or 12 x 1-mL vials

Download the BD^®OMICS-Guard Sample Preservation Buffer datasheet to learn how the BD^®OMICS-Guard SPB is used in CITE-seq or flow cytometry.

*BD^® OMICS-Guard SPB can be used to preserve single cells prior to nuclei isolation for ATAC-Seq Assays. We do not recommend preserving nuclei samples with BD^® OMICS-Guard SPB for ATAC-Seq Assays.

One-Step Sample Preservation Workflow

	Single-cell suspension	Tissue	Whole blood
Recommended usage	10,000 to 10 million cells per 1 mL BD^® OMICS-Guard SPB	30 to 50 mg of tissue per 20 mL BD^® OMICS-Guard SPB	1:1 Ratio of whole blood (with EDTA) and BD^® OMICS-Guard SPB
Storage temperature	4 °C
Storage duration	Up to 72 hours
Buffer handling	BD^® OMICS-Guard SPB should be used with aseptic techniques to prevent contamination of the stock buffer. We recommend working under a laminar flow hood
Sample preservation protocol	Collect single cells/dissociated single cells in suspension and spin at 400 x g for 5 min. Discard supernatant and resuspend the cells in BD^®OMICS-Guard SPB as recommended above. Place cells at 4 °C for up to 72 h. After storage, spin cells at 800 x g for 5 min and discard the supernatant to remove the BD^® OMICS-Guard SPB. No further washing is required. Resuspend the cells in desired buffer for downstream applications.	Cut tissue into 30–50 mg pieces and immediately place them into the BD^® OMICS-Guard SPB. Place the preserved tissue at 4 °C for up to 72 h. After storage, dissociate the tissue by the desired method for your single-cell application. Resuspend the cells in desired buffer for downstream applications.	Collect whole blood using EDTA as an anti-coagulant and mix with the same volume of BD^® OMICS-Guard SPB by inverting 10 times. Note: It may be possible to use other anti-coagulants, though they have not been tested. Place the whole blood and BD^® OMICS-Guard SPB mixture at 4 °C for up to 72 h. After storage, isolate desired cells. Leukocytes: use a magnetic red blood cell depletion kit to remove the red blood cells and isolate PBMCs and granulocytes. PBMCs: use density gradient separation methods (e.g., STEMCELL SepMate™ PBMC Isolation Tubes). Spin the isolated cells at 200 x g for 7 min to remove platelets. Resuspend cells in desired buffer for downstream applications.
Modifications to single-cell capture on the BD Rhapsody^™ Single-Cell Analysis System	For cells/tissues/whole blood preserved in BD^® OMICS-Guard SPB, ensure that the lysis time in the single-cell capture workflow is at least 5 min (can be longer per the specific assay instruction).

Improve Ab-Oligo Signal with the BD^® AbSeq Enhancer Kit for BD^® OMICS-Guard SPB-Preserved Samples in CITE-seq

If staining with antibody-oligo cocktails after BD^® AbSeq Antibody-Oligos after BD^® OMICS-Guard Buffer preservation, use the BD^® AbSeq Enhancer Kit to reduce nonspecific binding events and enhance the AbSeq signal.

Heatmaps of AbSeq performance with and without the addition of the BD^® AbSeq Enhancer Kit. Heatmaps of AbSeq log10(median molecules per cell) expression for different cell types are shown when the BD^®AbSeq Enhancer Kit is either not added to the protocol (top) or added to the protocol (bottom) when using preserved cells. Nonspecific background signal (top right) is eliminated, leading to better signal/noise, with the use of the BD^® AbSeq Enhancer Kit (bottom right), which closely matches that of the control data (bottom left).

Download the BD^® OMICS-Guard Sample Preservation Buffer product information sheet to access BD^® AbSeq Enhancer Kit staining protocols

Applications

BD^® OMICS-Guard Sample Preservation Buffer preserves cell viability, transcriptomic and proteomic profiles, and cell populations in human PBMCs for CITE-seq analyses

A time course CITE-seq analysis was conducted on the BD Rhapsody™ Single-Cell Analysis System with non-preserved (control) human PBMCs (0 h) and BD^® OMICS-Guard Buffer-preserved human PBMCs at 24, 48 and 72 h. PBMCs at each time point were stained with a 30-plex BD^® AbSeq Ab-Oligo Panel. Cell viability, gene expression, surface protein expression and the cell population in the preserved PBMCs at 24, 48 and 72 h were analyzed and compared to that of the control PBMC sample (n = 2)

OMICS-Guard-Preserved-vs-Nonpreserved-Gene-Expression-Correlation

OMICS-Guard-Preserved-vs-Nonpreserved-Protein-Correlation

BD^® OMICS-Guard Sample Preservation Buffer preserves cell viability, transcriptome and proteome profiles, and cell populations in mouse spleen tissue for CITE-seq analyses

A time course CITE-seq analysis was conducted on the BD Rhapsody™ Single-Cell Analysis System with non-preserved (control) mouse spleen tissues (0 h) and BD^® OMICS-Guard Buffer-preserved mouse spleen tissues at 24, 48 and 72 h. Dissociated mouse splenocytes at each time point were stained with a 30-plex BD^® AbSeq Ab-Oligo Panel. Cell viability, gene expression, surface protein expression and the cell population in preserved mouse spleen tissues at 24, 48 and 72 h were analyzed and compared to that of control mouse spleen tissues (n = 2).

OMICS-Guard-Mouse-Spleen-Protein-Expression

Mouse-Splenocyte-AbSeq-Sensitivity-Median-Molecules

BD^® OMICS-Guard Sample Preservation Buffer preserves transcriptomic and proteomic profiles and cell populations in human whole blood samples for CITE-seq analyses

A time course CITE-seq analysis was conducted on the BD Rhapsody^™ Single-Cell Analysis System with human whole blood samples collected in EDTA. PBMC and granulocytes were isolated from a portion of the whole blood sample by red blood cell depletion. An aliquot of these cells was immediately processed and not preserved, serving as a 0 h time point. The remaining extracted cells were mixed with BD^® OMICS-Guard SPB and stored at 4 ^°C for 24, 48, 72 hours (noted in graphs below as “cells”). The rest of the whole blood sample was then directly preserved in BD^® OMICS-Guard SPB by combining the sample with BD^® OMICS-Guard SPB at 1:1 ratio and stored for 24, 48 and 72 h at 4 °C. PBMC and granulocyte isolation was performed by red blood cell depletion at each of the 24, 48 and 72 h preservation time points (noted in graphs below as “WB”). At each time point, cells were stained with the 30-plex BD^® OMICS-One Immune Profiler Protein Panel. Whole transcriptome expression, cell surface protein expression and cell type distributions were analyzed at all time points to evaluate the performance of BD^® OMICS-Guard SPB preservation with whole blood.

Whole Blood BD OMICS Guard SPB Preservation

BD OMICS Guard Whole Blood Human Granulocyte Preservation

BD^® OMICS-Guard Sample Preservation Buffer preserves surface protein epitopes and cell populations in human PBMCs for flow cytometry analyses

A time course flow cytometry analysis was conducted using non-preserved (control) human PBMCs (0 h) and BD^® OMICS-Guard Buffer-preserved human PBMCs at 24, 48 and 72 h (n = 2). Human PBMCs at each time point were stained with a 13-color fluorescence antibody panel for major immune cell population identification and protein expression analyses and control (0 h) and preserved (24, 48 and 72 h) human PBMCs were compared.

omics-guard/applications/slide-3/b/Non-preserved-and-Preserved-Human-PBMCs-Protein-Marker

BD^® OMICS-Guard Sample Preservation Buffer preserves protein information in mouse spleen tissue

A time-course flow cytometry analysis was conducted using non-preserved (control) mouse spleen tissues (0 h) and BD^® OMICS-Guard Buffer-preserved mouse spleen tissues at 24, 48 and 72 h (n = 2). Dissociated mouse splenocytes at each time point were stained with an 11-color fluorescence antibody panel for major splenic cell population identification and protein expression analyses and control (0 h) and preserved (24, 48 and 72 h) mouse spleen tissues were compared. The protein expression level and frequencies of major cell populations revealed by flow cytometry analyses in mouse splenocytes stayed consistent over the course of 72 hours, indicating the capability of BD^® OMICS-Guard Preservation Buffer to preserve surface protein epitopes and cell composition for flow cytometry analyses.

omics-guard/applications/slide-4/a/Mouse-Spleen-Tissue-SPB-Protein-Preservation

Mouse-Spleen-Consistent-Key-Protein-Marker-Expresseion

Mouse-Spleen-CD45-Major-Leucocyte-Cell-Population-Consistent-Frequencies

Stress-free sample preservation with BD^® OMICS-Guard Sample Preservation Buffer demonstrated by expression analysis of 84 stress-associated genes in human PBMCs using qPCR

Expression of 84 stress-associated genes were compared between fresh and preserved human PBMC samples using qPCR (n = 2). No significant expression changes of stress-associated genes were observed after 48 h of preservation, indicating a stress-free preservation workflow with BD^® OMICS-Guard Preservation Buffer.