Targeted Kits

Overview

The BD Rhapsody™ Targeted mRNA Kits provide the ability to focus on specific targets for single-cell research using the BD Rhapsody™ Single-Cell Analysis System. Drawing from BD Biosciences’ 45+ years of expertise in immunology, our targeted kits comprise markers of interest to researchers. Designed to work with BD^®AbSeq and BD^® Single-Cell Multiplexing Kits, our targeted assays enable you to perform multiomic analyses while focusing on key genes of interest.

We offer four different gene panels to support targeted analyses:

Human Immune Response Targeted Panel (Features slide 1)

Mouse Immune Response Targeted Panel (Features slide 2)

Onco-BC Targeted Panel (Features slide 3)

Human T-Cell Targeted Panel (Features slide 4)

Read our app note to see how you can enable single-cell CRISPR screening using the BD Rhapsody^™ System.

Features

The BD Rhapsody™ Human Immune Response Targeted Panel enables cost-efficient cellular phenotyping of single cells, saves sequencing costs and analysis time, and allows better utilization of resources

Utilizes multiplex PCR to detect 399 genes chosen for profiling human immune cells
Focuses on genes relevant to phenotyping human immune cells
Includes genes involved in cytokine and chemokine secretion and interleukin signaling; and transcription factors involved in cell differentiation
and maturation
Allows customization by adding up to 100 user-defined genes
Specifically amplifies transcripts most relevant to immune-cell function rather than constitutively expressed ribosomal genes
Requires significantly fewer reads and improves assay efficiency

Find more information from the BD Rhapsody™ Human Immune Response Targeted Panel Brochure.

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The BD Rhapsody™ Mouse Immune Response Targeted Panel enables cellular phenotyping of single cells of murine origin and allows you to focus on key genes of interest at significantly lowered sequencing costs

Utilizes multiplex PCR to detect 397 genes chosen for profiling mouse immune cells.
Targets genes critical for phenotyping mouse immune cells.
Includes genes involved in cytokine and chemokine secretion and interleukin signaling; and transcription factors involved in cell differentiation and maturation

Find more information from the BD Rhapsody™ Mouse Immune Response Targeted Panel Brochure.

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The BD Rhapsody™ Onco-BC Targeted Panel is customizable and was created via collaborations with clinical oncologists

Utilizes multiplex PCR to detect 389 genes chosen for profiling human cancer-associated genes
Focuses on human breast cancer biology and includes genes with known involvement in cancer progression, proliferation, epithelial-to-mesenchymal transition (EMT) and cancer stem cells
Aids molecular subtyping of breast cancer cells with the inclusion of PAM50 genes
Enables comprehensive surveys of the complex tumor microenvironment due to the inclusion of stromal cell markers and immunophenotyping
Requires dramatically fewer reads and reduces time and sequencing costs

Find more information from the BD Rhapsody™ Onco-BC Targeted Panel Brochure.

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The BD Rhapsody™ Human T-Cell Targeted Panel provides a snapshot of T-cell gene expression to complement traditional techniques like FACS and offers a streamlined assay for single-cell T-cell research

Utilizes multiplex PCR to detect 259 genes chosen for T-cell profiling
Focuses on genes relevant to T-cell biology
Yields single-cell profile data that complements traditional FACS data

Find more information from the BD Rhapsody™ Human T-Cell Targeted Panel Brochure.

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Applications

The BD Rhapsody™ Human Immune Response Targeted Panel provides resolution of naïve, effector and memory CD4+ and CD8+ T-cell subsets

Example data of the BD Rhapsody™ Human Immune Response Targeted Panel

A. t-SNE projection of 3,201 PBMCs, annotated by cell type.

B. Box plots of marker gene expression for each major cell type.

The BD Rhapsody™ Mouse Immune Response Targeted Panel provides resolution of immune cell subsets, such as follicular, germinal center and marginal zone B cells, as well as MM/MZ and red pulp macrophages

Example data of the BD Rhapsody™ Mouse Immune Response Targeted Panel

A. t-SNE projection of 1,179 cells derived from mouse spleen, annotated by cell type.

B. Box plots of marker gene expression for each major cell type.

The BD Rhapsody™ Onco-BC Targeted Panel enables the detection of subpopulations of breast cancer cells and a diverse tumor microenvironment of stromal and major immune cell types from a metastatic breast cancer sample

Example data from analysis of a lymph node containing metastatic BRCA cells using the BD Rhapsody™ Onco-BC Targeted Panel

A. t-SNE projection of 4,409 cells annotated by cell type.

B. Box plots of marker gene expression for each major cell type show complex tumor heterogeneity.

The BD Rhapsody™ Human T-Cell Targeted Panel enables the identification of rare cell populations, such as gamma delta T cells, in addition to commonly observed CD4 and CD8 naïve, effector and memory T-cell populations

Example data of the BD Rhapsody™ T-cell Panel

A. t-SNE projection of 3,436 resting CD3+ T-cells, annotated by cell type.

B. Box plots of marker gene expression for each major cell type annotated in A.

C. t-SNE projection of 5,115 activated CD3+ T-cells, annotated by cell type.

D. Box plots of marker gene expression for each major cell type annotated in C.