Overview
We accelerate discovery and early-stage development with smarter biomarker identification, faster CAR-T assay development and standardized workflows across sites—from preanalytical through analytical and postanalytical phases.
Whether you’re uncovering new biomarkers, validating therapeutic targets or advancing translational studies, the BD integrated cell therapy solutions portfolio is engineered to streamline your discovery pipeline and set a stronger foundation for downstream development and manufacturing success.
BD Solutions for Cell Therapy Discovery and Early-Stage Development
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Preanalytical phase: Build stronger assays from the start
- Achieve consistent, high-quality performance panels on the BD FACSDiscover™ A8 Cell Analyzer and BD FACSDiscover™ S8 Cell Sorter with RUO and GMP-grade antibodies conjugated to best-in-class BD Horizon RealYellow™ and RealBlue™ Reagents —delivering reduced cross-laser spillover, robust intracellular performance and excellent resolution of diverse antigen densities.
- Characterize therapeutic candidates earlier using high-throughput single-cell multiomics that simultaneously profile RNA, protein and the immune repertoire, enabling more informed target prioritization and faster iteration.
- Achieve consistent, high-quality performance panels on the BD FACSDiscover™ A8 Cell Analyzer and BD FACSDiscover™ S8 Cell Sorter with RUO and GMP-grade antibodies conjugated to best-in-class BD Horizon RealYellow™ and RealBlue™ Reagents —delivering reduced cross-laser spillover, robust intracellular performance and excellent resolution of diverse antigen densities.
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Analytical phase: Reveal deeper biology, faster
Gain more insights into cellular heterogeneity to guide process refinement, strengthen decision-making and support more informed, data driven development across discovery and translational workflows. The BD FACSDiscover™ A8 Cell Analyzer and BD FACSDiscover™ S8 Cell Sorter featuring BD CellView™ Image Technology provides X 2 more imaging parameters than traditional flow cytometry enabling deeper characterization with the lowest cost-per-insight in flow.
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Postanalytical phase: Accelerate decision-making across teams
Increase collaboration and shorten time to insight with a robust software that enables cross-functional teams to review, analyze and interpret complex datasets with greater speed and confidence.
Expand Your Discovery Potential with a New Dimension
The BD FACSDiscover™ A8 Cell Analyzer and BD FACSDiscover™ S8 Cell Sorter provide the analytical depth indispensable to cell therapy advancement by combining state-of-the-art BD SpectralFX™ Technology with the spatial and functional insights delivered by real-time imaging using BD CellView™ Image Technology.
BD is redefining single‑cell analysis by combining the power of spectral flow cytometry with real‑time imaging—delivering groundbreaking integration that accelerates breakthroughs, enhances productivity and drives deeper scientific discovery.
Unlock New Biomarkers with BD Horizon™ Dyes
BD Horizon RealYellow™, RealBlue™, RealRed™ and RealViolet™ Reagents (BD Horizon™ Real Fluorochromes | BD Real Dyes) are engineered to reduced cross-laser spillover and optimize resolution when used with other fluorochromes, helping to expand experiments on both conventional and spectral flow cytometers.
Our enhanced BD Horizon™ Chroma Reagent technology supports high-parameter dried panels which can incorporate 10+ BD Horizon Brilliant™ Reagents and unlimited reagents in tube or plate format for high-throughput discovery workflows.
Elevate Biomarker Discovery with Seamless Multiomics Integration
BD’s single‑cell multiomics solution empowers researchers to expand biomarker discovery with simultaneous RNA and protein profiling through CITE‑seq, delivering comprehensive insights from each individual cell. Its gentle microwell‑based, bead‑enabled technology minimizes cell stress while enabling high‑throughput, cost‑effective studies. Combined with BD AbSeq reagents, the BD Rhapsody™ Scanner and advanced analysis software, the workflow offers a streamlined, end‑to‑end approach for generating high‑dimensional data that accelerates discovery across immune and tumor cell populations
Transform Data into Discovery with BD Data Analysis Solutions
FlowJo™ Software is the trusted choice for tens of thousands of scientists worldwide for their flow cytometry data analysis needs. It delivers a versatile analysis environment designed to help you reveal deeper insights from your data.
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Ultra-high Parameter Cell Analysis to Support Biomarker Discovery
BD SpectralFX™ Technology on the BD FACSDiscover™ Family of Instruments enabled the first 50-marker flow cytometry panel. These systems can resolve more than 50 fluorochromes simultaneously in real time, supporting both high-parameter cell analysis and cell sorting. This allows users to analyze complex cellular populations with unprecedented depth.
Konecny AJ, Mage P, Tyznik AJ, Prlic M, Mair F. OMIP-102: 50-color phenotyping of the human immune system with in-depth assessment of T cells and dendritic cells. Cytometry. 2024; 105(56): 430-436.
Assessing Activation Marker Distribution
Accelerate CAR-T Assay Development
The BD FACSDiscover™ Platform advances CAR-T assay development by enabling faster insights, streamlining steps and enhancing both workflow efficiency and data quality.
CAR-T Workflow, Process and Products
Accelerate Discovery with Power of Real-Time Insights
The BD FACSDiscover™ A8 Cell Analyzer and BD FACSDiscover™ S8 Cell Sorter provide the analytical depth indispensable to cell therapy advancement by combining state-of-the-art spectral flow cytometry with the spatial and functional insights delivered by real-time imaging using BD CellView™ Image Technology. Accelerate your development and improve your decision making by enabling workflows that could not be achieved previously.
BD® CAR Detection: Designed for your CAR-T Workflow
BD® CAR Detection Reagents uniquely combined with industry-leading fluorochromes and a ready-to-use format, to enable sensitive, rapid and reproducible detection even at very low CAR-T cell frequencies. Available for BCMA and CD19, BD® CAR Detection Reagents feature a variety of formats for maximum panel design flexibility.
Data‑Driven Performance Insights
The BD® BCMA CAR-T Detection Reagent empowers reliable and precise detection of CAR expression, enabling confident assessment of transduction efficiency, therapeutic potency, and overall product consistency. When paired with BD CellView™ Image Technology on BD FACSDiscover™ Platform it further reveals nuanced, cell-level differences in CAR BCMA expression—unlocking deeper insights that help accelerate and elevate assay development.
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Advance CAR‑T Development with Accurate BCMA Detection and Spatial Insight on Every Cell
Standardize Cell Therapy Development Across Sites
BD solutions deliver trusted instruments and reagents to ensure consistent, high-quality data across platforms, enabling seamless workflows, minimizing variability and maximizing confidence in every discovery.
Out-of‑the-Box Standardization for Confident Cell Analysis
The BD FACSDiscover™ A8 Cell Analyzer and BD FACSDiscover™ S8 Cell Sorter provide the perfect platform for robust, standardized and reproducible analysis, screening and sorting. Expect out-of-the box consistency in the data acquired at default gains: day-to-day and instrument-to-instrument, for all your assays.
Reagents Engineered for Confidence in Every Experiment
BD Horizon RealViolet™, RealBlue™, RealYellow™ and RealRed™ Reagents provide lot-to-lot consistency across made-to-stock and BD OptiBuild™ Reagents, with proven photostability under typical lab lighting—so your results stay accurate and reproducible.
Standardized Custom Panels for Confident, Multi‑Site Cell Therapy Studies
Standardization is critical for long-term studies and multi-site clinical trials to ensure data continuity and reliability. BD Horizon™ Chroma Reagent Panels provide ready-to-use dried cocktails that enable reproducibility and streamline workflows across trial sites.
Power Collaboration and Productivity Across Your Flow Cytometry Lab
BD® Research Cloud unifies your flow cytometry workflow in a single digital environment—making it easier to design panels, organize experiments, manage data and collaborate across teams. With tools to store and share instrument configurations, it helps labs stay organized, accelerate analysis and work more efficiently. Integrated remote instrument‑health monitoring further supports smooth operations, enabling your lab to scale its capabilities with confidence.
Robust, out‑of‑the‑box consistency across BD FACSDiscover™ A8 Instruments
Explore the inter‑instrument reproducibility of a 24‑color deep immunophenotyping panel designed to resolve complex T‑cell subsets, including highly co‑expressed markers and challenging chemokine receptors. When run across three independent BD FACSDiscover™ A8 Cell Analyzer instruments, the UMAP projection of the full‑color dataset shows exceptional agreement across all systems. Whether viewed individually or as an overlay, each dataset was acquired using default instrument settings, with no user adjustments required, demonstrating the platform’s robust consistency and reliable performance out‑of‑the‑box.
Seamlessly move from analysis to sorting
Take your analysis seamlessly from the BD FACSDiscover™ A8 Cell Analyzer to the BD FACSDiscover™ S8 Cell Sorter for downstream applications. The 50‑color (OMIP‑102) panel was transferred between instruments without any additional setup adjustments, demonstrating strong concordance across populations and MFI. This alignment is evident whether viewed through traditional bivariate scatter plots or UMAP projections with color‑identified clusters. The UMAP overlay further confirms the high level of agreement between the analyzer and sorter, reinforcing the platform’s reliability and cross‑instrument consistency.
Consistent, verified performance of BD Horizon™ Chroma Dried Panels
Our team works closely with customers to identify key quality specifications for each custom panel.
Our standard quality control process involves flow testing the dried cocktail in comparison with the liquid cocktail and prior reference batches.
Consistent performance over 3 batches of a standard T‑cell panel, dried and run on lysed whole blood.
| Tube Name | CD45+ %Parent | CD45+ %Median | CD3+ %Parent | CD3+ %Median | CD4+ %Parent | CD4+ %Median | CD8+ %Parent | CD8+ Median |
|---|---|---|---|---|---|---|---|---|
| Liquid | 34.5 | 19948 | 64.3 | 751 | 47.2 | 8027 | 42 | 6588 |
| BD Horizon™ Chroma Lot 1 | 31.1 | 19165 | 62.9 | 745 | 45.4 | 7697 | 42.2 | 6516 |
| BD Horizon™ Chroma Lot 2 | 31.9 | 19165 | 64.1 | 757 | 47.2 | 8355 | 41.4 | 6531 |
| BD Horizon™ Chroma Lot 3 | 29 | 18720 | 64.2 | 728 | 45.2 | 8355 | 43.7 | 6488 |
BD Horizon™ RB824 (RB824) Reagents Demonstrate Lot-to-Lot Consistency Across Made-to-Stock and BD OptiBuild™ On-Demand Reagents
RB824 offers stable performance with lot-to-lot consistency across made-to-stock reagents and BD OptiBuild™ On-Demand Reagents. Human whole blood was stained with human CD3 (UCHT1, left) or CD4 (SK3, right) RB824 using three different dye lots, followed by lysis with BD Pharm Lyse™ Lysing Buffer. All specificities were run on a Cytek™ Aurora Spectral Analyzer. CD3 and CD4 were tested in separate experiments.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
BD Flow Cytometers are Class I Laser Products.