BD^® ELISPOT Reagents

For the analysis of cytokines at the single-cell level.

Overview

The BD^®ELISPOT Assay is a powerful tool for detecting and analyzing individual cells that secrete a particular protein in vitro. BD^®ELISPOT Reagents coupled with a high-throughput ELISPOT plate reader provide intuitive analysis of cytokines at the single-cell level.

The reagents:

Provide direct analysis of frequency of cells producing cytokines and other effector molecules using an enzyme-linked immunospot (ELISPOT) assay
Offer greater sensitivity than ELISAs and intracellular staining
Enable high-throughput analyses for direct ex vivo monitoring of T-cell function, including antigen-specific T cells
Offer the flexibility to choose among several formats:
- BD^® ELISPOT Kits—ready-to-use kits with precoated BD^® ELISPOT Plates for human, nonhuman primate and mouse research
- BD^® ELISPOT Reagent Sets—coat-it-yourself sets with sufficient capture antibody, detection antibody and streptavidin-horseradish peroxidase for 10 plates worth of assays (includes 10 uncoated plates)
- BD^® ELISPOT Reagent Pairs–matched antibody pairs. Sufficient capture antibody and detection antibody for 5 plates

Features

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The BD^® ELISPOT Assay is able to detect cytokine release at the single-cell level, allowing for direct determination of cytokine-producing cell frequencies

The assay:

Has been found to be more sensitive than ELISAs and intracellular staining. The sensitivity of the assay enables measurement of very low frequencies of cytokine-producing cells (for example, 1 in 300,000).
Enables single-cell and high-throughput analyses for direct ex vivo monitoring of T-cell function, including antigen-specific T cells.
Contains carefully screened reagents to ensure the generation of crisp, clearly defined immunospots. High-affinity BD Pharmingen™ NA/LE Capture Antibodies safeguard against nonspecific cellular activation.
Has carefully selected detection antibodies, along with ready-to-use preparations of streptavidin-horseradish peroxidase, substrate and chromogen to produce optimal BD^®ELISPOT Reagents.

An automated coating process for the BD^® ELISPOT Plates helps to minimize inconsistency of results

BD Biosciences kits undergo strict quality control processes to ensure high-quality assay results with minimal well-to-well and plate-to-plate variation. The plates are coated with no azide/low endotoxin capture antibodies, are UV processed, and are tested and found negative for bacteria and fungi.