Purified Mouse Anti-Dystrobrevin

BD Transduction Laboratories™ Purified Mouse Anti-Dystrobrevin

Name: Purified Mouse Anti-Dystrobrevin
Brand: Purified Mouse Anti-Dystrobrevin
SKU: 610766

Clone 23/Dystrobrevin (RUO)

Western blot analysis of Dystrobrevin on rat brain lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of anti-Dystrobrevin.

Immunofluorescent staining of Rabbit Cerebrum.

Western blot analysis of Dystrobrevin on rat brain lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of anti-Dystrobrevin.

Immunofluorescent staining of Rabbit Cerebrum.

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Scientific Resources

제품 세부 정보

Brand: BD Transduction Laboratories™

Reactivity: Rat (QC Testing), Mouse, Rabbit (Tested in Development)

Isotype: Mouse IgG1

Immunogen: Mouse Dystrobrevin aa. 249-403

Application: Western blot (Routinely Tested), Immunohistochemistry (Tested During Development), Immunofluorescence, Immunoprecipitation (Not Recommended)

Target Molecular Weight: 87 kDa

Concentration: 250 µg/ml

RRID: AB_398089

Storage Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.

Regulatory Status: RUO

준비 및 보관

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

권장 분석 절차

Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml .

제품 고시

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.

610766 Rev. 1

항체 세부 정보

23/Dystrobrevin

Acetylcholine receptor clustering is considered an early event and a marker for synaptogenesis between a motor neuron and a muscle fiber. This process is mediated by the extracellular protein Agrin which binds to α and β Dystroglycan, previously discovered as Dystrobrevin- associated proteins. Dystrobrevin binds to the actin cytoskeleton by its N-terminal domain, thus providing the link between the actin cytoskeleton and receptor clustering. In Torpedo electric organ, Dystrobrevin is a prominent phosphotyrosine-containing protein. Murine Dystrobrevins comprise a family of four related proteins (1-4) with similar function, generated by alternative splicing. Dystrobrevin-1 is 688 amino acids and has an apparent mobility of 87kDa. Dystrobrevin-2 shares almost identical sequence with Dystrobrevin-1 in the first 500 amino acids and has an apparent mobility of 59kDa.

610766 Rev. 1

형광 세부 정보

Purified

Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.

형광: Purified

610766 Rev.1

인용 및 참고 문헌

View product citations for antibody "610766" on CiteAb

개발 참고 자료 (1)

Blake DJ, Nawrotzki R, Peters MF, Froehner SC, Davies KE. Isoform diversity of dystrobrevin, the murine 87-kDa postsynaptic protein. J Biol Chem. 1996; 271(13):7802-7810. (Biology). 참조 보기

610766 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.