Purified Mouse Anti-Cathepsin D

BD Transduction Laboratories™ Purified Mouse Anti-Cathepsin D

Name: Purified Mouse Anti-Cathepsin D
Brand: Purified Mouse Anti-Cathepsin D
SKU: 610801

Clone 49/Cathepsin D (RUO)

Western blot analysis of Cathepsin D on HepG2 cell lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000, dilution of anti-Cathepsin D.

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제품 세부 정보

Brand: BD Transduction Laboratories™

Reactivity: Human (QC Testing)

Isotype: Mouse IgG2a

Immunogen: Human Cathepsin D

Application: Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry, Immunoprecipitation (Not Recommended)

Target Molecular Weight: 43/28 kDa

Concentration: 250 µg/ml

RRID: AB_398120

Storage Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.

Regulatory Status: RUO

준비 및 보관

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

권장 분석 절차

Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml.

제품 고시

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.

610801 Rev. 2

항체 세부 정보

49/Cathepsin D

Cathepsin D, an enzyme that degrades proteins, was originally cloned during the search for estrogen-responsive genes in MCF-7 cells. Cathepsin D is synthesized as the 43kDa preprocathepsin D that is cleaved to form a 46kDa glycosylated procathepsin D. Procathepsin is then processed into 44kDa active Cathepsin D. The active and mature form undergoes further cleavage that yields 28kDa and 15kDa (heavy and light chains, respectively) fragments in SDS-PAGE. The heavy and light chains of Cathepsin D are released into the extracellular medium. The maturation process of Cathepsin D occurs through the transit from the endoplasmic reticulum, Golgi apparatus, to the lysosomes. Estrogen stimulates cell proliferation in a number of tumor cell lines and anti-estrogen therapy is often used in the treatment of breast cancer patients. Therefore, Cathepsin D, which is estrogen-inducible, may have a role during the pathogenesis of breast tumors. Additionally, several other roles have been proposed for this enzyme, such as tissue remodeling, tumor invasion, and embryo implantation.

610801 Rev. 2

형광 세부 정보

Purified

Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.

형광: Purified

610801 Rev.2

인용 및 참고 문헌

View product citations for antibody "610801" on CiteAb

개발 참고 자료 (4)

Erickson AH, Conner GE, Blobel G. Biosynthesis of a lysosomal enzyme. Partial structure of two transient and functionally distinct NH2-terminal sequences in cathepsin D. J Biol Chem. 1981; 256(21):11224-11231. (Biology). 참조 보기
Faust PL, Kornfeld S, Chirgwin JM. Cloning and sequence analysis of cDNA for human cathepsin D. Proc Natl Acad Sci U S A. 1985; 82(15):4910-4914. (Biology). 참조 보기
Kageyama T, Takahashi K. A cathepsin D-like acid proteinase from human gastric mucosa. Purification and characterization. J Biochem (Tokyo). 1980; 87(3):725-735. (Biology). 참조 보기
Westley BR, May FE. Oestrogen regulates cathepsin D mRNA levels in oestrogen responsive human breast cancer cells. Nucleic Acids Res. 1987; 15(9):3773-3786. (Biology). 참조 보기

모두 보기 (4)

610801 Rev. 2

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.