Purified Mouse Anti-Human EGF Receptor

BD Pharmingen™ Purified Mouse Anti-Human EGF Receptor

Name: Purified Mouse Anti-Human EGF Receptor
Brand: Purified Mouse Anti-Human EGF Receptor
SKU: 555996

Clone EGFR.1 (RUO)

Profile of A431, an epidermoid carcinoma cell line, analyzed on a FACScan (BDIS, San Jose, CA).

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제품 세부 정보

Brand: BD Pharmingen™

Reactivity: Human (QC Testing)

Isotype: Mouse IgG2b, κ

Application: Flow cytometry (Routinely Tested), Fluorescence microscopy (Tested During Development)

Concentration: 0.5 mg/ml

RRID: AB_2096589

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

준비 및 보관

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

제품 고시

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

관련 제품

FITC Goat Anti-Mouse IgG/IgM RUO

사이즈 0.5 mg 카탈로그 No 555988

Purified Mouse IgG2b κ Isotype Control RUO

사이즈 0.1 mg 카탈로그 No 555740

555996 Rev. 5

항체 세부 정보

EGFR.1

The EGFR.1 monoclonal antibody specifically binds to the epidermal growth factor receptor (EGF-R). The EGF-R is a transmembrane glycoprotein of approximately 170 kDa that is expressed on most cells. It consists of a glycosylated extracellular domain which binds to epidermal growth factor (EGF), a transmembrane domain, and an intracellular domain with tyrosine-kinase activity essential for signal transduction. The EGF-R plays an important role in the growth and differentiation of many cellular types. Transforming growth factor α (TGFα), vaccinia virus growth factor, and related growth factors can also bind to and signal through the EGF-R.

555996 Rev. 5

형광 세부 정보

Purified

Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.

형광: Purified

555996 Rev.5

인용 및 참고 문헌

View product citations for antibody "555996" on CiteAb

개발 참고 자료 (4)

Callard R, Gearing A. Callard R, Gearing A. The Cytokine Facts Book. San Diego: Academic Press; 1994.
Fernandez A, Spitzer E, Perez R. A new monoclonal antibody for detection of EGF-receptors in western blots and paraffin-embedded tissue sections. J Cell Biol. 1992; 49(2):157-165. (Biology). 참조 보기
Messa C, Russo F, Notarnicola M, Di Leo A. Demonstration of epidermal growth factor receptor in colorectal adenocarcinoma by enzyme immunoassay. Digestion. 1994; 55(2):103-107. (Biology). 참조 보기
Troalen F, Maudelondé T, Montcourrier P, Bohuon C, Delarue JC. Characterization of two monoclonal antibodies against the COOH-terminal part of the human epidermal growth factor receptor and potential clinical use. J Immunol Methods. 1991; 143(2):175-186. (Biology). 참조 보기

모두 보기 (4)

555996 Rev. 5

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.