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제품 세부 정보
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BD Pharmingen™
IL12RB2; Interleukin-12 receptor beta-2 chain
Human (QC Testing)
Rat IgG2a, κ
Human IL-12Rβ2 Recombinant Protein
Flow cytometry (Routinely Tested), Fluorescence microscopy (Reported)
20 µl
AB_393851
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


준비 및 보관

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
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제품 고시

  1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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550723 Rev. 4
항체 세부 정보
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2B6/12beta2

The 2B6 monoclonal antibody specifically recognizes the Interleukin 12 Receptor beta 2 subunit (IL-12Rβ2). IL-12Rβ2 serves as the signaling subunit that combines with CD212 (Interleukin 12 Receptor beta 1 subunit/ IL-12Rβ1) to form the functional receptor for IL-12. The IL-12Rβ2 is expressed on T cells and NK cells. IL-12R is a glycoprotein member of the hemopoietin receptor superfamily and is related to gp130, the granulocyte-colony stimulating factor (G-CSF) receptor and leukemia inhibitory factor (LIF). IL-12 is an immunomodulatory cytokine produced mainly by antigen presenting cells (APC) and plays a role in promoting Th1-type responses and cell mediated immunity. It stimulates proliferation of T cells and NK cells, enhances lytic activity of lymphokine activated (LAK) cells and cytolytic T cells (CTL).

550723 Rev. 4
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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PE
496 nm, 566 nm
576 nm
550723 Rev.4
인용 및 참고 문헌
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View product citations for antibody "550723" on CiteAb

개발 참고 자료 (4)

  1. Chizzonite R, Truitt T, Podlaski FJ, et al. IL-12: monoclonal antibodies specific for the 40-kDa subunit block receptor binding and biologic activity on activated human lymphoblasts. J Immunol. 1991; 147(5):1548-1556. (Biology). 참조 보기
  2. Chua AO, Chizzonite R, Desai BB, et al. Expression cloning of a human IL-12 receptor component. A new member of the cytokine receptor superfamily with strong homology to gp130. J Immunol. 1994; 153(1):128-136. (Biology). 참조 보기
  3. Presky DH, Minetti LJ, Gillessen S, et al. Analysis of the multiple interactions between IL-12 and the high affinity IL-12 receptor complex. J Immunol. 1998; 160(5):2174-2179. (Biology). 참조 보기
  4. Wu CY, Warrier RR, Carvajal DM, et al. Biological function and distribution of human interleukin-12 receptor beta chain. Eur J Immunol. 1996; 26(2):345-350. (Biology). 참조 보기
모두 보기 (4) 간단히 보기
550723 Rev. 4

Please refer to Support Documents for Quality Certificates

 

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

 

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.