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      Purified Mouse Anti-Human ErbB2
      E19420_610161_image1.png

      Western blot analysis ErbB2 on a A431 cell lysate (Human epithelial carcinoma; ATCC CRL-1555).  Lane 1: 1:2500, lane 2: 1:5000, lane 3: 1:10,000 dilution of the Mouse Anti-Human ErbB2 antibody.

      E19420_610161_image2.png

      Immunofluorescence staining of A431 cells (Human epithelial carcinoma; ATCC CRL-1555).

      E19420_610161_image1.png E19420_610161_image2.png Blue-Cap-White-Label.jpg

      Western blot analysis ErbB2 on a A431 cell lysate (Human epithelial carcinoma; ATCC CRL-1555).  Lane 1: 1:2500, lane 2: 1:5000, lane 3: 1:10,000 dilution of the Mouse Anti-Human ErbB2 antibody.

      Immunofluorescence staining of A431 cells (Human epithelial carcinoma; ATCC CRL-1555).

      제품 세부 정보
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      BD Transduction Laboratories™
      Neu, Her-2
      Human (QC Testing)
      Mouse IgG2b
      Rat ErbB2 aa. 182-373
      Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry, Immunoprecipitation (Tested During Development)
      185 kDa
      250 µg/ml
      AB_397563
      Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
      RUO


      준비 및 보관

      Store undiluted at -20°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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      제품 고시

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      610162 Rev. 2
      항체 세부 정보
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      42/c-erbB-2

      ErbB2 (Neu or Her-2) is a member of the erbB family of growth factor receptors. These factors possess constitutive tyrosine kinase activity and are commonly overexpressed in breast and ovarian carcinomas. While other erbB family members' ligands, such a EGF and NDF, are well characterized, a natural ligand for erbB2 remains unknown. ErbB2 forms heterodimers with erbB1/EGFR, erbB3, and erbB4, and can modulate their ligand affinities. Thus, erbB2 alters the intracellular responses elicited by EGF and NDF. This control is due to the fact that erbB2, when in complex with another erbB family receptor, decelerates the rate of ligand dissociation. Therefore, erbB2 may act as a signaling subunit for other receptors rather than a true growth factor receptor. Due to high sequence homology, this antibody may cross-react with the 180 kDa EGFR.

      610162 Rev. 2
      형광 세부 정보
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      Purified
      Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
      Purified
      610162 Rev.2
      인용 및 참고 문헌
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      View product citations for antibody "610162" on CiteAb

      개발 참고 자료 (5)

      1. Dillon C, Creer A, Kerr K, Kumin A, Dickson C. Basolateral targeting of ERBB2 is dependent on a novel bipartite juxtamembrane sorting signal but independent of the C-terminal ERBIN-binding domain. Mol Cell Biol. 2002; 22(18):6553-6563. (Biology: Western blot). 참조 보기
      2. Graus-Porta D, Beerli RR, Hynes NE. Single-chain antibody-mediated intracellular retention of ErbB-2 impairs Neu differentiation factor and epidermal growth factor signaling. Mol Cell Biol. 1995; 15(3):1182-1191. (Biology). 참조 보기
      3. Piechocki MP, Pilon SA, Wei WZ. Complementary antitumor immunity induced by plasmid DNA encoding secreted and cytoplasmic human ErbB-2. J Immunol. 2001; 167(6):3367-3374. (Biology: Flow cytometry, Immunofluorescence). 참조 보기
      4. Szollosi J, Balazs M, Feuerstein BG, Benz CC, Waldman FM. ERBB-2 (HER2/neu) gene copy number, p185HER-2 overexpression, and intratumor heterogeneity in human breast cancer. Cancer Res. 1995; 55(22):5400-5407. (Biology). 참조 보기
      5. Xu W, Mimnaugh E, Rosser MF. Sensitivity of mature Erbb2 to geldanamycin is conferred by its kinase domain and is mediated by the chaperone protein Hsp90. J Biol Chem. 2001; 276(5):3702-3708. (Biology: Western blot). 참조 보기
      모두 보기 (5) 간단히 보기
      610162 Rev. 2

      Please refer to Support Documents for Quality Certificates

       

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

       

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.