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      Purified Mouse Anti-Cyclin D3
      C28620_610279_image1.png

      Western blot analysis of Cyclin D3 on RSV-3T3 lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the Cyclin D3 antibody.

      C28620_610279_image2.png

      Immunofluorescent staining of Human Endothelial cells.

      C28620_610279_image1.png C28620_610279_image2.png Blue-Cap-White-Label.jpg

      Western blot analysis of Cyclin D3 on RSV-3T3 lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the Cyclin D3 antibody.

      Immunofluorescent staining of Human Endothelial cells.

      제품 세부 정보
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      BD Transduction Laboratories™
      Mouse (QC Testing), Human, Rat, Dog (Tested in Development)
      Mouse IgG2b
      Human Cyclin D3 aa. 127-292
      Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry, Immunoprecipitation (Tested During Development)
      33 kDa
      250 µg/ml
      AB_397674
      Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
      RUO


      준비 및 보관

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.
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      제품 고시

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
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      610279 Rev. 2
      항체 세부 정보
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      1/Cyclin D3

      Several classes of cyclins (A-E) have been described. These proteins act as regulatory subunits for cyclin-dependent kinases (cdks). The synthesis and degradation of cyclins is tightly controlled in a cell cycle specific manner. There are at least 3 different D-type cyclins whose relative levels vary among cell types. Cyclin D3 is known to associate with Cdk5 and weakly with Cdk2. Cdk4 is preferentially bound by Cyclin D1. However, in T cells which do not express Cyclin D1, Cdk4 associates with Cyclins D2 and D3. While bound to cdks, the D-type cyclins also associate with the polymerase-δ subunit, PCNA. Cyclin D3 has also been shown to bind pRb in vitro. This suggests that pRb may be an in vivo substrate of D-type cyclins.

      This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

      610279 Rev. 2
      형광 세부 정보
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      Purified
      Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
      Purified
      610279 Rev.2
      인용 및 참고 문헌
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      View product citations for antibody "610279" on CiteAb

      개발 참고 자료 (5)

      1. Bagui TK, Jackson RJ, Agrawal D, Pledger WJ. Analysis of cyclin D3-cdk4 complexes in fibroblasts expressing and lacking p27(kip1) and p21(cip1). Mol Cell Biol. 2000; 20(23):8748-8757. (Clone-specific: Immunoprecipitation, Western blot). 참조 보기
      2. Haller K, Wu Y, Derow E, Schmitt I, Jeang KT, Grassmann R. Physical interaction of human T-cell leukemia virus type 1 Tax with cyclin-dependent kinase 4 stimulates the phosphorylation of retinoblastoma protein. Mol Cell Biol. 2002; 22(10):3327-3338. (Clone-specific: Western blot). 참조 보기
      3. Motokura T, Keyomarsi K, Kronenberg HM, Arnold A. Cloning and characterization of human cyclin D3, a cDNA closely related in sequence to the PRAD1/cyclin D1 proto-oncogene. J Biol Chem. 1992; 267(28):20412-20415. (Biology). 참조 보기
      4. Saitoh H, Pizzi MD, Wang J. Perturbation of SUMOlation enzyme Ubc9 by distinct domain within nucleoporin RanBP2/Nup358. J Biol Chem. 2002; 277(7):4755-4763. (Clone-specific: Immunofluorescence). 참조 보기
      5. Xiong Y, Menninger J, Beach D, Ward DC. Molecular cloning and chromosomal mapping of CCND genes encoding human D-type cyclins. Genomics. 1992; 13(3):575-584. (Biology). 참조 보기
      모두 보기 (5) 간단히 보기
      610279 Rev. 2

      Please refer to Support Documents for Quality Certificates

       

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

       

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.