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Western blot analysis of PKCε on rat brain lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of PKCε.
PKC epsilon (clone 21) staining on rat brain. Formalin fixed paraffin section with citrate buffer pretreatment. 40X
BD Transduction Laboratories™ Purified Mouse Anti-PKCε
BD Transduction Laboratories™ Purified Mouse Anti-PKCε
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- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
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The Protein Kinase C (PKC) family of homologous serine/threonine protein kinases is involved in a number of processes such as growth, differentiation, and cytokine secretion. At least eleven isozymes have been described. These proteins are products of multiple genes and alternative splicing. PKC consists of a single polypeptide chain containing four conserved regions (C) and five variable regions (V). The N-terminal half containing C1, C2, V1, and V2 constitutes the regulatory domain and interacts with PKC activators Ca2], phospholipid, diacylglycerol, or phorbol ester. However, the novel PKC (nPKC) subfamily members (δ,η, η, and θ isoforms) and the atypical PKC (aPKC) subfamily members (ζ , ι , and λ isoforms) are Ca[2+] independent and lack the C2 domain. The PKC pathway represents a major signal transduction system that is activated following ligand-stimulation of transmembrane receptors by hormones, neurotransmitters and growth factors. Expression of the 90 kDa PKCα is induced by interferon-α. Generally, PKCα is expressed at very low levels in normal murine tissues, except brain. Overexpression of PKCα leads to increased growth rates and higher cell densities in monolayer cultures. A high level of expression is seen in several hematopoietic cell lines and tumors. This suggests a possible role for PKCα in tumorigenesis.
개발 참고 자료 (5)
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Balafanova Z, Bolli R, Zhang J. Nitric oxide (NO) induces nitration of protein kinase Cepsilon (PKCepsilon ), facilitating PKCepsilon translocation via enhanced PKCepsilon -RACK2 interactions: a novel mechanism of no-triggered activation of PKCepsilon. J Biol Chem. 2002; 277(17):15021-15027. (Clone-specific: ELISA, Immunoprecipitation, Western blot). 참조 보기
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Mischak H, Kolch W, Goodnight J. Expression of protein kinase C genes in hemopoietic cells is cell-type- and B cell-differentiation stage specific. 1999; 147(11):3981-3987. (Biology). 참조 보기
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Ohno S, Akita Y, Hata A. Structural and functional diversities of a family of signal transducing protein kinases, protein kinase C family; two distinct classes of PKC, conventional cPKC and novel nPKC. Adv Enzyme Regul. 1991; 31:287-303. (Biology). 참조 보기
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Ping P, Song C, Zhang J. Formation of protein kinase C(epsilon)-Lck signali. J Clin Invest. 2002; 109(4):499-507. (Clone-specific: Immunoprecipitation, Western blot). 참조 보기
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Zhu DM, Fang WH, Narla RK, Uckun FM. A requirement for protein kinase C inhibition for calcium-triggered apoptosis in acute lymphoblastic leukemia cells. Clin Cancer Res. 1999; 5(2):355-360. (Clone-specific: Immunofluorescence). 참조 보기
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