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- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Phosphatidylinositol 3 (PI3) -kinase participates in insulin-stimulated glucose uptake, PDGF-induced membrane ruffling, and G-protein receptor signaling. It exists as a heterodimer of 85 kDa (p85) and 110 kDa (p110) subunits. The p85 subunit associates with and serves as a substrate for activated growth factor receptor tyrosine kinases. p85 regulates the p110 catalytic subunit by acting as the link between PI3-kinase and the ligand-activated receptor. Four isoforms of p110 have been identified (α, β, γ, and δ). The p110α isoform contains an N-terminal region involved in p85 binding and a C-terminal kinase domain. p85/p110α-type PI kinase phosphorylates the D-3 and D-4 position of the inositol ring of PI, thereby producing PtdIns(3)P, PtdIns(3,4)P[2], PtdIns(3,4,5)P[3], PtdIns(4)P, and PtdIns(4,5) P[2]. During induction of chemotaxis by the chemokine SDF-1α, PI3-kinase regulates adhesion and ERM protein redistribution in the lymphocyte plasma membrane. In addition, PI3-kinases activate other signaling molecules, such as p70 S6 kinase and Akt/protein kinase B. Thus, p85/p110α-type PI kinase is a ubiquitously expressed kinase that is involved in a variety of cell signaling cascades.
개발 참고 자료 (5)
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Funaki M, Katagiri H, Kanda A. p85/p110-type phosphatidylinositol kinase phosphorylates not only the D-3, but also the D-4 position of the inositol ring. J Biol Chem. 1999; 274(31):22019-22024. (Biology). 참조 보기
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Hu Q, Klippel A, Muslin AJ, Fantl WJ, Williams LT. Ras-dependent induction of cellular responses by constitutively active phosphatidylinositol-3 kinase. Science. 1995; 268(5207):100-102. (Biology). 참조 보기
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Katagiri H, Asano T, Ishihara H. Overexpression of catalytic subunit p110alpha of phosphatidylinositol 3-kinase increases glucose transport activity with translocation of glucose transporters in 3T3-L1 adipocytes. J Biol Chem. 1996; 271(29):16987-16990. (Biology). 참조 보기
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Vicente-Manzanares M, Rey M, Jones DR. Involvement of phosphatidylinositol 3-kinase in stromal cell-derived factor-1 alpha-induced lymphocyte polarization and chemotaxis. J Immunol. 1999; 163(7):4001-4012. (Biology). 참조 보기
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Volinia S, Hiles I, Ormondroyd E. Molecular cloning, cDNA sequence, and chromosomal localization of the human phosphatidylinositol 3-kinase p110 alpha (PIK3CA) gene. Genomics. 1994; 24(3):472-477. (Biology). 참조 보기
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