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Western blot analysis of phosphotyrosine on A431 cell lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of anti-phosphotyrosine, PY20.
BD Transduction Laboratories™ Purified Mouse Anti-Phosphotyrosine
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제품 고시
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Phosphorylation of specific tyrosine residues is the result of of activation or stimulation or stimulation of their respective protein tyrosine kinases. The phosphorylated proteins can be autophosphorylated kinases or certain cellular protein substrates that are regulated in oncogenesis or cell growth. Antibodies to phosphotyrosine provide one of the best tools for the detection and characterization of phosphotyrosine proteins.
Technical Note: The use of milk-containing buffers may interfere with a phosphotyrosine antibody's ability to bind specific proteins of interest. Please use BSA-containing buffers for blocking and incubating purposes.
This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
개발 참고 자료 (5)
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Baisden JM, Gatesman AS, Cherezova L, Jiang BH, Flynn DC. The intrinsic ability of AFAP-110 to alter actin filament integrity is linked with its ability to also activate cellular tyrosine kinases. Oncogene. 2001; 20(45):6607-6616. (Clone-specific: Immunofluorescence). 참조 보기
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Glenney JR Jr, Zokas L, Kamps MP. Monoclonal antibodies to phosphotyrosine. J Immunol Methods. 1988; 109(2):277-285. (Biology). 참조 보기
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Lund-Johansen F, Davis K, Bishop J, de Waal Malefyt R. Flow cytometric analysis of immunoprecipitates: high-throughput analysis of protein phosphorylation and protein-protein interactions. Cytometry. 2000; 39(4):250-259. (Clone-specific: Flow cytometry). 참조 보기
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Vinos J, Freeman M. Evidence that Argos is an antagonistic ligand of the EGF receptor. Oncogene. 2000; 19(31):3560-3562. (Clone-specific: Western blot). 참조 보기
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Yamakawa N, Tsuchida K, Sugino H. The rasGAP-binding protein, Dok-1, mediates activin signaling via serine/threonine kinase receptors. EMBO J. 2002; 21(7):1684-1694. (Clone-specific: Immunoprecipitation, Western blot). 참조 보기
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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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