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- BD® AbSeq Assay
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Functional Assays
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Microscopy and Imaging Reagents
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Cell Preparation and Separation Reagents
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- BD® AbSeq Assay
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- BD® Single-Cell Multiplexing Kit
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- BD Rhapsody™ Whole Transcriptome Analysis (WTA) Amplification Kit
- BD Rhapsody™ TCR/BCR Profiling Assays
- BD OMICS-Guard Sample Preservation Buffer
- BD Rhapsody™ ATAC-Seq Assays
- BD Rhapsody™ TCR/BCR Next Multiomic Assays
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Western blot analysis of CLA-1 on a HeLa cell lysate (Human cervical epitheloid carcinoma; ATCC CCL-2.2). Lane 1: 1: 500, lane 2: 1:1000, lane 3: 1:2000 dilution of the mouse anti-human CLA-1 antibody.
Immunofluorescence staining of human endothelial cells.
BD Pharmingen™ Purified Mouse Anti-Human CLA-1
BD Pharmingen™ Purified Mouse Anti-Human CLA-1
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준비 및 보관
권장 분석 절차
Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml
제품 고시
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
관련 제품
CLA-1 (CD36 and LIMPII Analogous-1) is member of a novel gene family that includes CD36, LIMPII, and SR-BI. CD36 is a cell surface glycoprotein that binds to collagen type I and thrombospondin. LIMPII (lysosomal integral membrane protein II), as its name suggests, is expressed on the membrane of lysosomes. SR-BI (scavenger receptor type B class I) is involved in the selective uptake of cholesterol esters. These proteins include two membrane-anchoring regions, two short cytoplasmic tails, and a large extracellular/luminal domain. CLA-1 mRNA is detected in a wide range of tissues including adrenal glands, liver, and testis. Its expression and similarity with other family members suggest it may play a role in HDL metabolism. However, identification of the CLA-1 receptor on monocytes indicates additional CLA-1 functions in leukocytes.
개발 참고 자료 (4)
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Acton S, Rigotti A, Landschulz KT, Xu S, Hobbs HH, Krieger M. Identification of scavenger receptor SR-BI as a high density lipoprotein receptor. Science. 1996; 271(5248):518-520. (Biology). 참조 보기
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Calvo D, Vega MA. Identification, primary structure, and distribution of CLA-1, a novel member of the CD36/LIMPII gene family. J Biol Chem. 1993; 268(25):18929-18935. (Biology). 참조 보기
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Kozarsky KF, Donahee MH, Rigotti A, Iqbal SN, Edelman ER, Krieger M. Overexpression of the HDL receptor SR-BI alters plasma HDL and bile cholesterol levels. Science. 1997; 387(6631):414-417. (Biology). 참조 보기
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Lasley RD, Narayan P, Uittenbogaard A, Smart EJ. Activated cardiac adenosine A(1) receptors translocate out of caveolae. J Biol Chem. 2000; 275(6):4417-4421. (Biology: Western blot). 참조 보기
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