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Western blot analysis of Cadherin-5 on human endothelial cell lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of anti-Cadherin-5 antibody.
Immunofluorescent staining of Human Fibroblasts with anti-Cadherin-5 antibody.
BD Transduction Laboratories™ Purified Mouse Anti-Cadherin-5
BD Transduction Laboratories™ Purified Mouse Anti-Cadherin-5
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권장 분석 절차
Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml.
제품 고시
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
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Cadherins are a family of transmembrane glycoproteins involved in the Ca2+- dependent cell-cell adhesion that occurs in many tissues. These proteins are similar in their domain structure (45-74% amino acid conservation), Ca2+ and protease-sensitivity, and molecular weight. Cadherin-5 (VE-Cadherin or CD144) is one of a number of cadherins (cadherin-4 through -11) whose cDNAs were isolated from rat brain and retina. These cadherins have a cytoplasmic domain that is highly conserved relative to previously identified cadherins, indicating that this domain is essential for cell adhesion activity. This function is mediated by cadherin interaction with cytoskeletal proteins. However, Cadherin-5's cytoplasmic domain has the lowest degree of homology with the other cadherins. Cadherin-5 is expressed in brain and various other tissues, including umbilical cord vein endothelial cells. A new type of adhering junction has been identified in certain vascular endothelial cells. These junctions are known as "complexus adherens" and are morphologically and compositionally distinct from desmosomes and zonula adherens junctions. The complexus adherens of endothelial cells lack desmosomal cadherins as well as E-Cadherin. However, these cells are rich in Cadherin-5 which colocalizes with desmoplakin and γ-Catenin (plakoglobin).
개발 참고 자료 (5)
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Corada M, Liao F, Lindgren M. Monoclonal antibodies directed to different regions of vascular endothelial cadherin extracellular domain affect adhesion and clustering of the protein and modulate endothelial permeability. Blood. 2001; 97(6):1679-1684. (Clone-specific: Immunofluorescence, Western blot). 참조 보기
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Corada M, Zanetta L, Orsenigo F. A monoclonal antibody to vascular endothelial-cadherin inhibits tumor angiogenesis without side effects on endothelial permeability. Blood. 2002; 100(3):905-911. (Clone-specific: Flow cytometry, Western blot). 참조 보기
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Rahimi N, Kazlauskas A. A role for cadherin-5 in regulation of vascular endothelial growth factor receptor 2 activity in endothelial cells. Mol Biol Cell. 1999; 10:3401-3407. (Clone-specific: Functional assay). 참조 보기
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Schmelz M, Franke WW. Complexus adhaerentes, a new group of desmoplakin-containing junctions in endothelial cells: the syndesmos connecting retothelial cells of lymph nodes. J Cell Biol. 1993; 61(2):274-289. (Biology). 참조 보기
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Suzuki S, Sano K, Tanihara H. Diversity of the cadherin family: evidence for eight new cadherins in nervous tissue. Cell Regul. 1991; 2(4):261-270. (Biology). 참조 보기
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