Purified Rat Anti-Mouse PNAd Carbohydrate Epitope

BD Pharmingen™ Purified Rat Anti-Mouse PNAd Carbohydrate Epitope

Name: Purified Rat Anti-Mouse PNAd Carbohydrate Epitope
Brand: BD Pharmingen™
SKU: 553863

Clone MECA-79

(RUO)

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제품 세부 정보

Brand: BD Pharmingen™

Product Name: Ms PNAd Carbo Epitope Pure MECA-79 500ug

Alternative Name: CD62L Ligand

Reactivity: Mouse (QC Testing)

Isotype: Rat WF, also known as Wistar Furth IgM, κ

Immunogen: Collagenase-dispersed BALB/c lymph node stroma

Application: Immunohistochemistry-paraffin (Routinely Tested), Blocking, Immunohistochemistry-frozen, Immunohistochemistry-zinc-fixed, Immunoprecipitation, Western blot (Reported)

Concentration: 0.5 mg/ml

RRID: AB_395099

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

준비 및 보관

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

권장 분석 절차

This antibody has been tested by immunohistochemical staining (IHC) of citrate-pretreated formalin-fixed paraffin-embedded sections (5 - 20 µg/ml) to assure specificity and reactivity. Other reported applications include IHC of acetone-fixed frozen sections, immunoprecipitation, western blot analysis, and in vitro and in vivo adhesion blocking.

제품 고시

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.

관련 제품

Purified Rat IgM, κ Isotype Control RUO

사이즈 0.5 mg 카탈로그 No 553940

FITC Goat Anti-Rat Ig RUO

사이즈 0.5 mg 카탈로그 No 554016

553863 Rev. 7

항체 세부 정보

MECA-79

The MECA-79 antibody reacts with sulfate-dependent carbohydrate epitopes of peripheral lymph node addressin (PNAd). The MECA-79-reactive antigen is closely associated with the carbohydrate ligands for L-selectin (eg, CD34, GlyCAM-1, MAdCAM-1), which are expressed on high endothelial venules (HEV) in lymphoid tissues and at sites of chronic inflammation. Cross-reactivity with human, sheep, cow, primate, and pig tissues has been observed. MECA-79 antibody inhibits L-selectin-dependent lymphocyte and platelet homing to lymph nodes in vivo, and in vitro adhesion to lymphoid tissue HEV and immobilized PNAd.

553863 Rev. 7

형광 세부 정보

Purified

Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.

형광: Purified

553863 Rev.7

인용 및 참고 문헌

개발 참고 자료 (9)

Berg EL, Robinson MK, Warnock RA, Butcher EC. The human peripheral lymph node vascular addressin is a ligand for LECAM-1, the peripheral lymph node homing receptor. J Cell Biol. 1991; 114(2):343-349. (Clone-specific: Blocking, Immunoprecipitation). 참조 보기
Binns RM, Whyte A, Licence ST. The role of E-selectin in lymphocyte and polymorphonuclear cell recruitment into cutaneous delayed hypersensitivity reactions in sensitized pigs. J Immunol. 1996; 157(9):4094-4099. (Biology). 참조 보기
Diacovo TG, Puri KD, Warnock RA, Springer TA, von Andrian UH. Platelet-mediated lymphocyte delivery to high endothelial venules. Science. 1996; 273(5272):252-255. (Clone-specific: Blocking). 참조 보기
Faveeuw C, Gagnerault MC, Lepault F. Expression of homing and adhesion molecules in infiltrated islets of Langerhans and salivary glands of nonobese diabetic mice. J Immunol. 1994; 152(12):5969-5978. (Biology). 참조 보기
Hemmerich S, Butcher EC, Rosen SD. Sulfation-dependent recognition of high endothelial venules (HEV)-ligands by L-selectin and MECA 79, and adhesion-blocking monoclonal antibody. J Exp Med. 1994; 180(6):2219-2226. (Clone-specific: Immunoprecipitation). 참조 보기
Malý P, Thall A, Petryniak B, et al. The alpha(1,3)fucosyltransferase Fuc-TVII controls leukocyte trafficking through an essential role in L-, E-, and P-selectin ligand biosynthesis. Cell. 1996; 86(4):643-653. (Biology). 참조 보기
Michie SA, Streeter PR, Bolt PA, Butcher EC, Picker LJ. The human peripheral lymph node vascular addressin. An inducible endothelial antigen involved in lymphocyte homing. Am J Pathol. 1993; 143(6):1688-1698. (Clone-specific: Blocking). 참조 보기
Puri KD, Finger EB, Gaudernack G, Springer TA. Sialomucin CD34 is the major L-selectin ligand in human tonsil high endothelial venules. J Cell Biol. 1995; 131(1):261-270. (Clone-specific: Blocking, Western blot). 참조 보기
Streeter PR, Rouse BT, Butcher EC. Immunohistologic and functional characterization of a vascular addressin involved in lymphocyte homing into peripheral lymph nodes. J Cell Biol. 1988; 107(5):1853-1862. (Immunogen: Blocking, Immunohistochemistry). 참조 보기

모두 보기 (9)

553863 Rev. 7

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.