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준비 및 보관
제품 고시
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Although hamster immunoglobulin isotypes have not been well defined, BD Biosciences Pharmingen has grouped Armenian and Syrian hamster IgG monoclonal antibodies according to their reactivity with a panel of mouse anti-hamster IgG mAbs. A table of the hamster IgG groups, Reactivity of Mouse Anti-Hamster Ig mAbs, may be viewed at http://www.bdbiosciences.com/documents/hamster_chart_11x17.pdf.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
관련 제품
The H22 antibody reacts with mouse interferon-γ (IFN-γ) , but reportedly not with mouse IFN-α , IFN-β , IL-6, or human IFN-γ. Although not routinely tested, the H22 antibody has been reported to inhibit the in vitro and in vivo activities of mouse IFN-γ. Specific binding of I-125 labeled recombinant mouse IFN-γ to IFN-γ receptor-positive cells has been reported to be blocked by H22. Although the H22 antibody is reported to react with rat IFN-γ , its reactivity with IFN-γ from other species has not been tested.
개발 참고 자료 (5)
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Bancroft GJ, Schreiber RD, Bosma GC, Bosma MJ, Unanue ER. A T cell-independent mechanism of macrophage activation by interferon-gamma. J Immunol. 1987; 139(4):1104-1107. (Clone-specific: Neutralization). 참조 보기
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Buchmeier NA, Schreiber RD. Requirement of endogenous interferon-gamma production for resolution of Listeria monocytogenes infection. Proc Natl Acad Sci U S A. 1985; 82(21):7404-7408. (Clone-specific: Neutralization). 참조 보기
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Green JA, Yeh TJ, Overall JC. Rapid, quantitative, semiautomated assay for virus-induced and immune human interferons. J Clin Microbiol. 1980; 12(3):433-438. (Methodology). 참조 보기
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Leiby DA, Fortier AH, Crawford RM, Schreiber RD, Nacy CA. In vivo modulation of the murine immune response to Francisella tularensis LVS by administration of anticytokine antibodies. Infect Immun. 1992; 60(1):84-89. (Clone-specific: Neutralization). 참조 보기
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Schreiber RD, Hicks LJ, Celada A, Buchmeier NA, Gray PW. Monoclonal antibodies to murine -interferon which differentially modulate macrophage activation and antiviral activity. J Immunol. 1985; 134(3):1609-1618. (Clone-specific: Neutralization). 참조 보기
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