준비 및 보관
권장 분석 절차
ELISA (IL-12 p70 Detection): The Biotin Mouse Anti-Human IL-12 (p40/p70) (Clone C8.6, Cat. No. 554660) antibody is useful as a detection antibody in sandwich ELISA for measuring human IL-12 (p70). Biotinylated C8.6 antibody can be paired with the Purified Rat Anti-Human IL-12 p70 mAb (Clone 20C2, Cat. No. 555065) as the capture antibody and recombinant human IL-12 (p70) protein (Cat. No. 554613) as the standard. For testing human IL-12 (p70) in complex biological fluids, such as serum or plasma, investigators are highly encouraged to use the BD OptEIA™ Human IL-12 (p70) ELISA Set (Cat. No. 555183).
ELISA (IL-12 p40 Detection): The Biotin Mouse Anti-Human IL-12 (p40/p70) (Clone C8.6, Cat. No. 554660) antibody is useful as a detection antibody in sandwich ELISA for measuring human IL-12 (p40). Biotinylated C8.6 antibody can be paired with the Purified Mouse Anti-Human IL-12 (p40) mAb (Clone C8.3, Cat. No. 551227) as the capture antibody with recombinant human IL-12 (p40) protein (Cat. No. 554633) as the standard. For testing human IL-12 (p40) in complex biological fluids, such as serum or plasma, investigators are highly encouraged to use the BD OptEIA™ Human IL-12 (p40) ELISA Set (Cat. No. 555171).
제품 고시
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
관련 제품
The C8.6 monoclonal antibody specifically recognizes human IL-12 p40 that is encoded by IL12B (interleukin 12B). The antibody binds to IL-12 p40 monomers and homodimers as well as to the p40 subunit of the heterodimeric cytokines, IL-12 p70 (p35/p40) and IL-23 (p19/p40). These cytokines are produced by a variety of cells, including activated monocytes, macrophages, neutrophils, and dendritic cells. IL-12 p40 monomers and homodimers can block the binding of heterodimeric IL-12 to its receptor. IL-12 promotes the generation of Th1 cells and activates NK cells, Group 1 innate lymphoid cells (ILC1), and cytotoxic T cells leading to production of IFN-γ, TNF, granzymes, and perforin. IL-23 promotes the generation and activation of Th17 cells and other effector cells, including γδ T cells, NKT cells, MAIT cells, and ILC3 that can produce cytokines including IL-17A, IL-17F, IL-22, GM-CSF, TNF, and IL-6. The immunogen used to generate the C8.6 hybridoma was the purified p40 subunit of human IL-12. The C8.6 antibody is a neutralizing antibody and can reportedly inhibit the biological activities of IL-12 and IL-23.
개발 참고 자료 (3)
-
D'Andrea A, Aste-Amezaga M, Valiante NM, Ma X, Kubin M, Trinchieri G. Interleukin 10 (IL-10) inhibits human lymphocyte interferon gamma-production by suppressing natural killer cell stimulatory factor/IL-12 synthesis in accessory cells. J Exp Med. 1993; 178(3):1041-1048. (Clone-specific: Neutralization). 참조 보기
-
D'Andrea A, Rengaraju M, Valiante NM, et al. Production of natural killer cell stimulatory factor (interleukin 12) by peripheral blood mononuclear cells. J Exp Med. 1992; 176(5):1387-1398. (Clone-specific: Neutralization). 참조 보기
-
Oppmann B, Lesley R, Blom B, et al. Novel p19 protein engages IL-12p40 to form a cytokine, IL-23, with biological activities similar as well as distinct from IL-12.. Immunity. 2000; 13(5):715-25. (Clone-specific: Immunoprecipitation). 참조 보기
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.