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      Biotin Mouse Anti-Human IgG1
      제품 세부 정보
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      BD Pharmingen™
      Hu IgG1 Biotin G17-1 500ug
      Human (QC Testing)
      Mouse IgG2b, κ
      ELISA (Routinely Tested)
      0.5 mg/ml
      AB_396187
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      준비 및 보관

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
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      권장 분석 절차

      This antibody can be used to detect human IgG1. For the sandwich human IgG1 ELISA, biotin G17-1 is optimal as detecting mAb with G18-145 (Cat. No. 555784) as capture mAb.

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      제품 고시

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
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      555869 Rev. 7
      항체 세부 정보
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      G17-1

      The G17-1 antibody reacts specifically with human IgG1. It does not react with other Ig isotypes.

      555869 Rev. 7
      형광 세부 정보
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      Biotin
      Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.
      Biotin
      555869 Rev.7
      인용 및 참고 문헌
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      개발 참고 자료 (1)

      1. Guesdon JL, Ternynck T, Avrameas S. The use of avidin-biotin interaction in immunoenzymatic techniques. J Histochem Cytochem. 1979; 27(8):1131-1139. (Methodology: ELISA). 참조 보기
      555869 Rev. 7

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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