Fluorochrome marker assignment for a 17-color spectral flow cytometry panel
| Specificity | Clone | Fluorochrome | |
|---|---|---|---|
| UV 355 nm | CD25 | M-A251 | |
CD56 | NCAM16.2 | ||
CD20 | L27 | ||
CD19 | SJ25C1 | ||
CD16 | 3G8 | ||
CD14 | M5E2 | ||
AutoF | N/A | N/A | |
TIM-3 | 7D3 | ||
CD4 | SK3 | ||
| Violet 405 nm | TNF | MAb11 | |
VPD450 | N/A | N/A | |
CD3 | UCHT1 | ||
CD134 | ACT35 | ||
| Blue 488 nm | IFNγ | B27 | |
CD69 | FN50 | ||
FVS620 | N/A | N/A | |
LAG-3 | T47-530 | ||
PD-1 | EH12.1 | ||
II-2 | MQ1-17H12 | ||
| Yellow-Green 561 nm | CTLA-4 | BNI3 | |
| Red 640 nm | GranzB | GB11 | |
CD8 | SK1 |
Fluorochrome marker assignment for a 17-color spectral flow cytometry panel acquired on the BD FACSymphony™ A5 SE Cell Analyzer.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.