The BD FACSDiscover™ A7 Cell Analyzer introduces a paradigm shift in the standardization of spectral flow cytometry. Powered by BD SpectralFX™ and FACSTruQ™ Technology, the BD FACSDiscover™ A7 Cell Analyzer sets a new benchmark for data reproducibility and data comparability, so you can generate high-quality data with confidence and ease.
Delivering value through:
- Robust data quality independent of cytometry expertise
- Consistent and reproducible performance across instruments, users, and sites
- Less time on setup and calibration, more time on your science
State-of-the-art Flow Cytometry
Advanced spectral performance with BD SpectralFX™ Technology combines full-spectrum optics, optimized hardware and system-aware unmixing algorithms to effectively deliver spectral resolution and sensitivity enabling you to resolve challenging markers.
- Expand your panels to higher parameters without compromising the data quality
- Choose dyes for panel building based on biology and science, not your instrument limitations
- Reveal more biology in complex samples, including multiple autofluorescence populations.
Trust Your Data Across Users, Instruments, Sites and Time
Industry-leading standardization with FACSTruQ™ Technology including Detector Setting Independent (DSI) scaling is enabled by next‑generation setup, QC and automated system calibration.
- Achieve data consistency and reproducibility across experiments, instruments, sites, and over time, with the default pre-optimized settings
- Ensure data comparability even when detector settings need adjustment in rare cases, with the DSI innovation
- Improve reproducibility across instruments and longitudinal studies without manual harmonization or sacrificing flexibility and sensitivity.
Move from Panel Design to Trusted Data Faster
Simpler and intuitive spectral workflow with BD FACSChorus™ Software and Integrated Autoloader, automate sample processing for walk-away throughput with less hands-on time, all while supporting 21 CFR Part 11 compliance:
- Achieve high-quality spectral flow cytometry data results regardless of user experience, with built-in tools.
- Process more samples with less hands-on time through automated sample loading, temperature control, automated mixing, low sample carryover, and automated shutdown.
- Bring new users up to speed in hours, not days, with the guided software workflow.
Across 56 CD4 single-stain samples evaluated on 12 BD FACSDiscover™ A7 Instruments, % Coefficient of Variation (CV) of Positive MFI were consistently low, demonstrating technical evidence of the system's reproducibility and stability at default settings.
Sample matched, cryopreserved PBMC's were stained with a 20 Color BD Horizon Chroma™ Dried Panel. The panel focused on T Cells, B Cells and Monocytes. Samples were acquired on 12 BD FACSDiscover™ A7 Instruments over the course of three days. The 20-color panel delivered consistent resolution across all 12 instruments, demonstrating strong system stability and reproducibility.
The resolution of the 20-color panel over the course of 45 days remained consistent, demonstrating longitudinal stability of the system over time. Variations in results reflect biological change and not instrument variation.
Detector Setting Independent (DSI) scaling is a feature unique to the BD FACSDiscover™ Platform that sets a new standard for flow cytometry standardization and enables consistent intra- and inter-instrument data scaling.
A high-parameter 50-color panel was run across three BD FACSDiscover™ A7 Systems using the same sample during a single afternoon to evaluate instrument-to-instrument reproducibility. Despite the complexity of the panel, cell populations remain highly consistent across instruments, demonstrating robust performance where reproducibility traditionally becomes most challenging. These results reinforce the ability of FACSTruQ™ Technology to deliver standardized, reproducible data across systems, even for advanced high-parameter experiments, providing confidence in data comparability.
Powered by DSI scaling, the BD FACSDiscover™ A7 Cell Analyzer sets higher standards for the future of standardization with the FACSTruQ™ Technology.
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