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Still Using PE Tandem Dyes? You Can Do Better!

BD Horizon RealYellow™ and BD Horizon RealBlue™ Reagents

 

Introducing BD Horizon RealYellow™ and BD Horizon RealBlue™ Reagents: The superior alternative to PE and PerCP tandem dyes

 

Our next-generation fluorochromes offer:

 

  • Reduced background: significant reduction in monocyte background compared to PE-Cy tandems
  • Range of brightness: a range of fluorochromes to support the detection of surface and intracellular markers from a variety of expression levels
  • Versatility: compatible with a variety of common fixation and permeabilization systems, viability dyes and anti-coagulants
  • Reliable performance: stable reagents that are photostable and offer lot-to-lot consistency
  • Panel design flexibility: use RealYellow and RealBlue Reagent Pairs together on appropriately equipped instruments to build larger panels and for added flexibility when designing panels on both conventional and spectral flow cytometers.
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Use the next-generation laser-specific flow cytometry research reagents driven by the new BD dye technology specifically engineered to overcome the challenges of PE tandems. See how:

FormatSpectralConventionalRelative BrightnessSpillover
(1-low, 4-high)
Alternative To
Blue Laser Line
RB545  1Unique position
RB613 2PE-CF594*, PE-Dazzle™ 594* or BB630-P2
RB705 2PerCP-Cy5.5 or BB700
RB744 1BB755-P
RB780 1PE-Cy7* or BB7990-P
Yellow-Green Laser Line
RY586 1PE**
RY610 1PE-CF594** or PE-Dazzle™ 594**
RY703 2PE-Cy5.5** or StarBright™ Yellow 720
RY775 2PE-Cy7** or StarBright™ Yellow 800
* On three-laser configuration (B, V, R)
** On four- or five-laser configuration (B, V, R, YG or UV, V, B, YG, R)

 

 

Compared to PE-Cy tandems, BD Horizon RealYellow™ and BD Horizon RealBlue™ Reagents offer reduced background.

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BD Horizon™ RB780 Reagents offer reduced background compared to PE-Cy7

RY775-Improved-nonspecific-Binding
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RY775 provides improved nonspecific binding.

Compared to PE and PerCP tandems, BD Horizon RealYellow™ and BD Horizon RealBlue™ Reagents offer significantly reduced spillover spread.

RB-780 Emission Profile
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RY610 shows reduced spillover spread into the corresponding blue detectors.

RB613-and-PE-CF594-Normalized-Emission-Profile
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RB613 shows reduced spillover spread into the corresponding yellow-green detectors.

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BD Horizon RealYellow™ 586 Reagents offer high resolution with minimal cross-laser excitation. Data was acquired on a BD FACSymphony™ A5 SE Cell Analyzer.

Unlike select PE tandems such as PE-Cy7, PE-Cy5.5, BD Horizon RealYellow™ and BD Horizon RealBlue™ Reagents offer the detection of low-expression surface and intracellular markers.

RY703-Reagent-Lower-Expression-Intracellular-Markers
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BD Horizon™ RY703 Reagent resolves low-expression intracellular markers

Cytokine-Marker
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BD Horizon™ RB780 Reagents support the detection of low-expression intracellular markers.

RY775-Resolves-Intracellular-Markers
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BD Horizon™ RY775 Reagent resolves intracellular markers

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Cy is a trademark of Global Life Sciences Solutions Germany GmbH or an affiliate doing business as Cytiva.