Purified Mouse Anti-P-Cadherin

BD Transduction Laboratories™ Purified Mouse Anti-P-Cadherin

Name: Purified Mouse Anti-P-Cadherin
Brand: Purified Mouse Anti-P-Cadherin
SKU: 610227

Clone 56/P-Cadherin (RUO)

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카탈로그 No:610227

단위:EA (1 Each)

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제품 세부 정보

Brand: BD Transduction Laboratories™

Reactivity: Human (QC Testing), Rat (Tested in Development)

Isotype: Mouse IgG1

Immunogen: Human P-Cadherin aa. 72-259

Application: Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry, Immunoprecipitation (Tested During Development)

Target Molecular Weight: 120 kDa

Concentration: 250 µg/ml

RRID: AB_2077667

Storage Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.

Regulatory Status: RUO

준비 및 보관

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

권장 분석 절차

Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml.

제품 고시

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.

데이터 시트

Technical Data Sheet (TDS)

Safety Data Sheet

610227 Rev. 1

항체 세부 정보

56/P-Cadherin

Cadherins are a family of transmembrane glycoproteins involved in the Ca2+-dependent cell-cell adhesion that occurs in many tissues. Cadherins are similar in their domain structure, Ca2+ and protease sensitivity, and molecular weight. However, these proteins have distinct tissue expression patterns and immunological reactivities. P-Cadherin is localized in placenta while E-Cadherin and N-Cadherin are found in epithelial and neural tissues, respectively. P-Cadherin is an 829 amino acid polypeptide with a putative signal peptide and precursor region, an extracellular domain containing several internal repeats, and a highly hydrophobic transmembrane region. The cytoplasmic domain provides a link to the cytoskeleton through the associated catenin proteins.

610227 Rev. 1

형광 세부 정보

Purified

Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.

형광: Purified

610227 Rev.1

인용 및 참고 문헌

View Development References

개발 참고 자료 (5)

Bailey T, Biddlestone L, Shepherd N, Barr H, Warner P, Jankowski J. Altered cadherin and catenin complexes in the Barrett's esophagus-dysplasia-adenocarcinoma sequence: correlation with disease progression and dedifferentiation. Am J Pathol. 1998; 152(1):135-144. (Clone-specific: Immunohistochemistry). 참조 보기
Holsinger LJ, Ward K, Duffield B, Zachwieja J, Jallal B. The transmembrane receptor protein tyrosine phosphatase DEP1 interacts with p120(ctn). Oncogene. 2002; 21(46):7067-7076. (Clone-specific: Western blot). 참조 보기
Kantak SS, Kramer RH. E-cadherin regulates anchorage-independent growth and survival in oral squamous cell carcinoma cells. J Biol Chem. 1998; 273(27):16953-16961. (Clone-specific: Western blot). 참조 보기
Nose A, Nagafuchi A, Takeichi M. Isolation of placental cadherin cDNA: identification of a novel gene family of cell-cell adhesion molecules. EMBO J. 1987; 6(12):3655-3661. (Biology). 참조 보기
Tao YS, Edwards RA, Tubb B, Wang S, Bryan J, McCrea PD. beta-Catenin associates with the actin-bundling protein fascin in a noncadherin complex. J Cell Biol. 1996; 134(5):1271-1281. (Clone-specific: Immunofluorescence). 참조 보기

모두 보기 (5)

610227 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.