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Western blot analysis of Nucleoporin p62 on HeLa cell lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of anti-Nucleoporin p62.
Immunofluorescent staining on WI38 cells.
BD Transduction Laboratories™ Purified Mouse Anti-Nucleoporin p62
BD Transduction Laboratories™ Purified Mouse Anti-Nucleoporin p62
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The nuclear pore complex (NPC) acts as a gate to mediate active transport of proteins and RNA into and out of the nucleus. Proteins actively transported into the nucleus through the NPC require specific nuclear localization sequences. Many of these nucleoporins contain N-acetylglucosamine (GlcNAc) residues that are O-linked to serine or threonine. p62 is the best characterized member of a group of nucleoporins that line the central region of the NPC. A tightly associated complex is formed by p62 and two other nucleoporins, p54 and p58. p54 binds to a carboxy-terminal coiled-coil domain of p62 and p58 binds to a dimer of p54. The amino-terminal domain of p62 contains a series of XFXFX repeats and is joined to the coiled-coil domain by a threonine-rich linker segment. The major role of p62 is maintenance of the structural integrity of NPCs.
개발 참고 자료 (5)
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Carmo-Fonseca M, Kern H, Hurt EC. Human nucleoporin p62 and the essential yeast nuclear pore protein NSP1 show sequence homology and a similar domain organization. Eur J Cell Biol. 1991; 55(1):17-30. (Biology). 참조 보기
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Daigle N, Beaudouin J, Hartnell L, et al. Nuclear pore complexes form immobile networks and have a very low turnover in live mammalian cells. J Cell Biol. 2001; 154(1):71-84. (Clone-specific: Immunohistochemistry). 참조 보기
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Iborra FJ, Jackson DA, Cook PR. The path of RNA through nuclear pores: apparent entry from the sides into specialized pores. J Cell Sci. 2000; 113(2):291-302. (Clone-specific: Electron microscopy). 참조 보기
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Paroni G, Henderson C, Schneider C, Brancolini C. Caspase-2 can trigger cytochrome C release and apoptosis from the nucleus. J Biol Chem. 2002; 277(17):15147-15161. (Clone-specific: Immunofluorescence). 참조 보기
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Shah M, Patel K, Fried VA, Sehgal PB. Interactions of STAT3 with caveolin-1 and heat shock protein 90 in plasma membrane raft and cytosolic complexes. Preservation of cytokine signaling during fever. J Biol Chem. 2002; 277(47):45662-45669. (Clone-specific: Western blot). 참조 보기
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