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Western blot analysis of Gephyrin on rat brain lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of anti-Gephyrin.
Rat cerebellum, formalin-fixed paraffin embedded tissue, with citrate pre-treatment, 20X.
BD Transduction Laboratories™ Purified Mouse Anti-Gephyrin
BD Transduction Laboratories™ Purified Mouse Anti-Gephyrin
Purified Mouse Anti-Gephyrin
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- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
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The sub-membraneous region of the postsynaptic neuron is an intricate network of cytoskeletal elements generally known as the postsynaptic density. It is thought that this elaborate cytoskeletal region is critical for receptor targeting, clustering, and efficient signal input. Gephyrin, a 93 kDa protein, was identified as a result of its ability to bind to polymerized tubulin (microtubules). Although expressed in all tissues, gephyrin mRNA is found most abundantly in brain. Alternative splicing produces at least five different mRNAs. Gephyrin colocalizes and interacts with the glycine receptor at the postsynaptic density. It is possible that Gephyrin acts as an anchor between the glycine receptor and microtubules.
This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
개발 참고 자료 (4)
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Feng G, Tintrup H, Kirsch J, et al. Dual requirement for gephyrin in glycine receptor clustering and molybdoenzyme activity. Science. 1998; 282(5392):1321-1324. (Clone-specific: Western blot). 참조 보기
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Kneussel M, Haverkamp S, Fuhrmann JC, et al. The gamma-aminobutyric acid type A receptor (GABAAR)-associated protein GABARAP interacts with gephyrin but is not involved in receptor anchoring at the synapse. Proc Natl Acad Sci U S A. 2000; 97(15):8594-8599. (Clone-specific: Immunofluorescence, Western blot). 참조 보기
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Meyer G, Kirsch J, Betz H, Langosch D. Identification of a gephyrin binding motif on the glycine receptor beta subunit. Neuron. 1995; 15(3):563-572. (Biology). 참조 보기
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Prior P, Schmitt B, Grenningloh G, et al. Primary structure and alternative splice variants of gephyrin, a putative glycine receptor-tubulin linker protein. Neuron. 1992; 8(6):1161-1170. (Biology). 참조 보기
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