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Western blot analysis of Annexin I on a human endothelial cell lysate. Lane 1: 1:5000, lane 2: 1:10,000, lane 3: 1:20,000 dilution of the mouse anti-Annexin I antibody.
Immunohistochemical staining of a rabbit cerebrum section.
BD Transduction Laboratories™ Purified Mouse Anti-Annexin I
BD Transduction Laboratories™ Purified Mouse Anti-Annexin I
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준비 및 보관
권장 분석 절차
Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml
제품 고시
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
관련 제품
Annexin I, also known as lipocortin-1 or calpactin II, is part of a calcium-dependent phospholipid-binding protein family. Members share a common core domain, but each has a unique N-terminal tail that imparts its functional specificity. It is believed that phosphorlyation of this region in annexin I regulates its functions. Annexin I has been implicated in regulating phospholipid vesicle aggregation, mediating inflammatory response, and inhibiting the activity of phospholipase A2. Additionally, it has been found that the 10 kDa protein S100C influences annexin I's activity by binding to the N-terminal 13 residues of annexin in a manner similar to that of the annexin II/p11 complex.
개발 참고 자료 (4)
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Chasserot-Golaz S, Vitale N, Sagot I, et al. Annexin II in exocytosis: catecholamine secretion requires the translocation of p36 to the subplasmalemmal region in chromaffin cells. J Cell Biol. 1996; 133(6):1217-1236. (Biology). 참조 보기
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Graham ME, Gerke V, Burgoyne RD. Modification of annexin II expression in PC12 cell lines does not affect Ca(2+)-dependent exocytosis. Mol Biol Cell. 1997; 8(3):431-442. (Biology). 참조 보기
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Hansen MD, Ehrlich JS, Nelson WJ. Molecular mechanism for orienting membrane and actin dynamics to nascent cell-cell contacts in epithelial cells. J Cell Biol. 2002; 277(47):45371-45376. (Biology: Immunofluorescence, Immunoprecipitation, Western blot). 참조 보기
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Regnouf F, Sagot I, Delouche B, et al. "In vitro" phosphorylation of annexin 2 heterotetramer by protein kinase C. Comparative properties of the unphosphorylated and phosphorylated annexin 2 on the aggregation and fusion of chromaffin granule membranes. J Biol Chem. 1995; 270(45):27143-27150. (Biology). 참조 보기
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