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제품 세부 정보
BD Pharmingen™
Igh-1a
Mouse (QC Testing)
Mouse SJL IgG2a, κ
Not reported
ELISA (Routinely Tested)
0.5 mg/ml
AB_394886
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
준비 및 보관
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
제품 고시
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
553501 Rev. 12
항체 세부 정보
8.3
The 8.3 antibody reacts specifically with mouse IgG2a of Igh-Ca and the d, e, f, g, h, j, n, and o haplotypes (eg, BALB/c, C58, and A, AKR, CBA, C3H/He, NZB). It does not react with IgG2a of Igh-Cb nor the c and p haplotypes (eg, C57BL/6, C57BL/10, SJL, DBA/1, DBA/2, SWR).
553501 Rev. 12
형광 세부 정보
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
553501 Rev.12
인용 및 참고 문헌
View product citations for antibody "553501" on CiteAb
개발 참고 자료 (2)
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Huang CM, Parsons M, Oi VT, Huang HJ, Herzenberg LA. Genetic characterization of mouse immunoglobulin allotypic determinants (allotopes) defined by monoclonal antibodies. Immunogenetics. 1983; 18(4):311-321. (Clone-specific). 참조 보기
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Parsons M, Oi VT, Huang CM, Herzenberg LA. Structural characterization of mouse immunoglobulin allotypic determinants (allotopes) defined by monoclonal antibodies. Immunogenetics. 1983; 18(4):323-334. (Clone-specific). 참조 보기
553501 Rev. 12
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.