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Perm/Wash Buffer I

BD Phosflow™ Perm/Wash Buffer I

(RUO)
제품 상세
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설명

BD Phosflow™ Perm/Wash Buffer I is intended to be used for the intracellular staining of post-translationally modified signaling proteins.  BD Phosflow™ Perm/Wash Buffer I is used to permeabilize cells and to serve as an antibody diluent and cell wash buffer.  It is optimized for use with the BD Phosflow™ brand of intracellular phosphorylated signaling protein-specific antibodies.  BD Phosflow™ Perm/Wash Buffer I is provided as a  10X concentrated solution containing FBS and saponin. The presence of a small amount of precipitate may be observable and will not affect the performance of the buffer.  Because saponin-mediated cell permeabilization is a reversible process, it is important to keep the cells in the presence of saponin during intracellular staining.  

BD Phosflow™
Aqueous buffered solution containing saponin, fetal bovine serum and ≤ 0.09% sodium azide.
RUO
AB_2869104
Intracellular staining (flow cytometry) (Routinely Tested)
Phosflow Perm/Wash Buffer I 125mL


권장 분석 절차

Flow cytometry: Dilute BD Phosflow™ Perm/Wash Buffer I  1:10 in distilled water prior to use. If desired, the diluted 1X BD™ Phosflow Perm/Wash Buffer I can be passed through a 0.45 µm filter to remove any residual precipitate.  The BD Phosflow™ Perm/Wash Buffer I can be used to permeabilize or wash cells and to dilute antibodies for immunofluorescent staining of intracellular proteins.

제품 안내

  1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
557885 Rev. 5
Citations & References
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Development References (6)

  1. Assenmacher M, Schmitz J, Radbruch A. Flow cytometric determination of cytokines in activated murine T helper lymphocytes: expression of interleukin-10 in interferon-gamma and in interleukin-4-expressing cells. Eur J Immunol. 1994; 24(5):1097-1101. (Biology). View Reference
  2. Elson LH, Nutman TB, Metcalfe DD, Prussin C. Flow cytometric analysis for cytokine production identifies T helper 1, T helper 2, and T helper 0 cells within the human CD4+CD27- lymphocyte subpopulation. J Immunol. 1995; 154(9):4294-4301. (Biology). View Reference
  3. Jung T, Schauer U, Heusser C, Neumann C, Rieger C. Detection of intracellular cytokines by flow cytometry. J Immunol Methods. 1993; 159(1-2):197-207. (Biology). View Reference
  4. Krutzik PO, Nolan GP. Intracellular phospho-protein staining techniques for flow cytometry: monitoring single cell signaling events. Cytometry A. 2003; 55(2):61-70. (Biology). View Reference
  5. Prussin C, Metcalfe DD. Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies. J Immunol Methods. 1995; 188(1):117-128. (Biology). View Reference
  6. Sander B, Andersson J, Andersson U. Assessment of cytokines by immunofluorescence and the paraformaldehyde-saponin procedure. Immunol Rev. 1991; 119:65-93. (Biology). View Reference
View All (6) View Less
557885 Rev. 5

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.