PE Mouse Anti-Mouse H-2Kb

Consider BD Horizon RealYellow™ 586 (RY586) Reagents, a bright and clean fluorochrome alternative to PE off the yellow-green laser. RY586 can be used alongside PE on spectral cytometers. More Info

BD Pharmingen™ PE Mouse Anti-Mouse H-2Kb

Name: PE Mouse Anti-Mouse H-2Kb
Brand: BD Pharmingen™
SKU: 553570

Clone AF6-88.5

(RUO)

Spectrum Viewer

Protocol Library

Scientific Resources

제품 세부 정보

Brand: BD Pharmingen™

Product Name: Ms H-2Kb PE AF6-88.5 100ug

Reactivity: Mouse (QC Testing)

Isotype: Mouse BALB/c IgG2a, κ

Immunogen: Mouse C57BL/6 Splenocytes

Application: Flow cytometry (Routinely Tested), Immunofluorescence (Tested During Development)

Concentration: 0.2 mg/ml

Entrez Gene ID: 14972

RRID: AB_394928

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

준비 및 보관

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

제품 고시

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

관련 제품

PE Mouse IgG2a, κ Isotype Control RUO

사이즈 0.1 mg 카탈로그 No 553457

553570 Rev. 11

항체 세부 정보

AF6-88.5

The AF6-88.5 antibody recognizes the H-2Kb MHC class I alloantigen. Reactivity with other haplotypes (e.g., d, f, j, k, p, q, r, s, u, v) has not been observed.

553570 Rev. 11

형광 세부 정보

R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

형광: PE

Excitation Source: Yellow-Green 488 nm, 532 nm, 561 nm

Excitation Max: 496 nm, 566 nm

Emission Max: 576 nm

553570 Rev.11

인용 및 참고 문헌

개발 참고 자료 (2)

Loken MR, Stall AM. Flow cytometry as an analytical and preparative tool in immunology. J Immunol Methods. 1982; 50(3):R85-R112. (Clone-specific). 참조 보기
Wall KA, Lorber MI, Loken MR, McClatchey S, Fitch FW. Inhibition of proliferation of MIs- and Ia-reactive cloned T cells by a monoclonal antibody against a determinant shared by I-A and I-E.. J Immunol. 1983; 131(3):1056-64. (Clone-specific). 참조 보기

553570 Rev. 11

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.