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Multicolor flow cytometric analysis of CCR9 (CD199) expression on human peripheral blood mononuclear cells. Human PBMCs were stained with Alexa Fluor™ 647 Mouse Anti-Human CD4 antibody (Cat. No. 557707) and PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; Left Plot) or PE Mouse Anti-Human CCR9 (CD199) antibody (Cat. No. 567695; Right Plot) at 1 μg/test. Bivariate pseudocolor density plots showing the correlated expression of CCR9 (CD199) [or Ig Isotype control staining] versus CD4 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Cell Analyzer System equipped with a Blue Laser. Data shown on this Technical Data Sheet are not lot specific.
BD Pharmingen™ PE Mouse Anti-Human CCR9 (CD199)
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권장 분석 절차
BD® CompBeads can be used as surrogates to assess fluorescence spillover (Compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.
제품 고시
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- An isotype control should be used at the same concentration as the antibody of interest.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
관련 제품
The C9Mab-1 monoclonal antibody specifically binds to Chemokine (C-C motif) receptor 9 (CCR9), which is also known as CD199. CCR9 is an ~42 kDa seven-transmembrane protein in the G protein-coupled receptor (GPCR) supergene family. It is upregulated by CD4+CD8+ double-positive thymocytes, then downregulated in committed CD4+ or CD8+ single-positive thymocytes. CCR9 is also expressed on small intestinal B cells, subsets of memory CD4+ T cells and CD8+ T cells, and dendritic cells. CCR9 binds CCL25, which is also known as thymus-expressed chemokine (TECK) or small inducible chemokine 25 (Scya25). CCL25 is highly expressed in the thymic cortex and small intestinal epithelium. CCR9 is involved in T cell development and gut-associated immune functions via lymphocyte recruitment to the gastrointestinal tract.
개발 참고 자료 (4)
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Olaussen RW, Farstad IN, Brandtzaeg P, Rugtveit J. Age-related changes in CCR9+ circulating lymphocytes: are CCR9+ naive T cells recent thymic emigrants?. Scand J Immunol. 2001; 54(5):435-9. (Biology). 참조 보기
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Papadakis KA, Prehn J, Nelson V, et al. The role of thymus-expressed chemokine and its receptor CCR9 on lymphocytes in the regional specialization of the mucosal immune system.. J Immunol. 2000; 165(9):5069-76. (Biology). 참조 보기
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Wurbel MA, Malissen B, Campbell JJ. Complex regulation of CCR9 at multiple discrete stages of T cell development. Eur J Immunol. 2006; 36(1):73-81. (Biology). 참조 보기
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Zabel BA, Agace WW, Campbell JJ, et al. Human G protein-coupled receptor GPR-9-6/CC chemokine receptor 9 is selectively expressed on intestinal homing T lymphocytes, mucosal lymphocytes, and thymocytes and is required for thymus-expressed chemokine-mediated chemotaxis.. J Exp Med. 1999; 190(9):1241-1256. (Biology). 참조 보기
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