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- BD Rhapsody™ TCR/BCR Next Multiomic Assays
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T-bet (O4-46)
About T-bet
ISOTYPE
IgG1, k
CYTOMETER USED
FACSCantoII
EXPERIMENT CELL SOURCE
Human PBMC (Fresh Blood)
REACTIVE SPECIES
Human, Mouse
HOST SPECIES
Mouse
NCBI ENTREZ INFO
T-bet V450
Perm I | Perm II | Perm III | Perm IV (0.5x) | Perm IV (1.0x) | |
---|---|---|---|---|---|
Lymphocytes |
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Perm I | Perm II | Perm III | Perm IV (0.5x) | Perm IV (1.0x) | |
---|---|---|---|---|---|
Lymphocytes | 2.68 | 3.39 | 3.5 | 3.1 | 3.08 |
T-bet A488
Perm I | Perm II | Perm III | Perm IV (0.5x) | Perm IV (1.0x) | |
---|---|---|---|---|---|
Lymphocytes |
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Perm I | Perm II | Perm III | Perm IV (0.5x) | Perm IV (1.0x) | |
---|---|---|---|---|---|
Lymphocytes | 3.09 | 3.94 | 4.06 | 3.43 | 3.38 |
T-bet PE
Perm I | Perm II | Perm III | Perm IV (0.5x) | Perm IV (1.0x) | |
---|---|---|---|---|---|
Lymphocytes |
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Perm I | Perm II | Perm III | Perm IV (0.5x) | Perm IV (1.0x) | |
---|---|---|---|---|---|
Lymphocytes | 3.6 | 4.25 | 4.33 | 3.78 | 3.72 |
T-bet PerCP-Cy5.5
Perm I | Perm II | Perm III | Perm IV (0.5x) | Perm IV (1.0x) | |
---|---|---|---|---|---|
Lymphocytes |
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Perm I | Perm II | Perm III | Perm IV (0.5x) | Perm IV (1.0x) | |
---|---|---|---|---|---|
Lymphocytes | 4.07 | 3.88 | 3.98 | 3.86 | 3.85 |
T-bet A647
Perm I | Perm II | Perm III | Perm IV (0.5x) | Perm IV (1.0x) | |
---|---|---|---|---|---|
Lymphocytes |
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Perm I | Perm II | Perm III | Perm IV (0.5x) | Perm IV (1.0x) | |
---|---|---|---|---|---|
Lymphocytes | 4.6 | 4.42 | 4.53 | 4.11 | 4.07 |

Perm Buffer IV 1X vs. 0.5X:
Perm Buffer IV (provided as a 10X stock solution) may be used at a 1X or 0.5X concentration. The higher (1X) concentration provides optimal resolution of certain intracellular phosphoprotein stains but might result in increased cell loss and decreased ability to stain certain cell surface markers.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Alexa Fluor, Pacific Blue and Texas Red are trademarks of Life Technologies Corporation. Cy is a trademark of Global Life Sciences Solutions Germany GmbH or an affiliate doing business as Cytiva. AmCyan is a trademark of Takarabio Inc. BD-40149 (v1.0) 0721