BD FACSDiscover™ Spectral Flow Cytometers with Real-time Imaging Parameters
Meet the BD FACSDiscover™ family of instruments, the world's first matched-pair spectral analyzer and sorter with real-time imaging. Equipped with state-of-the-art BD SpectralFX™ and revolutionary BD CellView™ Image Technologies, these cytometers redefine biological research. BD SpectralFX™ Technology offers unparalleled panel design flexibility, while BD CellView™ Technology expands into spatial and morphological dimensions. This powerful combination enables scientists to visualize the previously unseen and tackle questions once deemed impossible. Step into a new era of scientific discovery with BD FACSDiscover™ cytometers.
BD FACSDiscover™ A8 Analyzer | BD FACSDiscover™ S8 Sorter |
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Applications for the BD FACSDiscover™ Platform
Isolate biologically relevant doublets
Real-time imaging can separate coincidental doublets from passive synapses and active synapse. Use the Radial Moment image parameter to identify doublets, and Delta Center of Mass and Maximum Intensity image parameters to identify accumulation of signal at the site of synapse. Radial Moment can also be used to remove doublets with higher confidence for alternative gating strategies.
Resolve sub-populations based on spatial distribution of markers
Gain sub-population clarity using real-time imaging to separate co-localized signal from dispersed signal. The Correlation image parameter is used to identified populations with high correlation of signals (such as the TNF treated population that has NFkB co-localized with the DNA stain in the nucleus) versus populations with low correlation of signals (such as the unstimulated populations with NFkB in the cytoplasm separate from the DNA in the nucleus).
Use label-free identification to separate populations
Regardless of whether you’re an environmental microbiologist sorting samples by stain-free morphology or an oncoimmunologist seperating immune cells from tumor cells based on size, using real-time label-free imaging can help cleanly separate populations that was not previously possible at flow cytometry speeds. Gain sub-population clarity using real-time imaging to separate co-localized signal from dispersed signal.
Level-Up Your Flow Cytometry with State-of-the-Art Spectral and Image Technology
The BD FACSDiscover™ A8 Analyzer and S8 Sorter matched-pair leverages BD SpectralFX™ Technology and BD CellView™ Image technology for exceptional data consistency with expanded spatial and morphological information.
Get data consistency with BD SpectralFX™ Technology
BD SpectralFX™ improves consistency and performance with a next-generation QC system, automated detector setup and a simplified spectral workflow.
See the Unseeable with BD CellView™ Image Technology
BD CellView™ Image Technology provides confidence, clarity and spatial information by integrating real-time imaging into your existing cytometry workflow.
The mouse-over feature of BD CellView™ Image Technology aids in determining the exclusion of debris and abnormal cells from the initial cell gate. The eccentricity (LL) vs radial moment (LL) plot at center is explored. Schematics along the axes of this plot show how these features measure different aspects of signal distribution. Typically, events with high eccentricity and high radial moment are doublets and clumps. An overlay of well-known scatter populations including lymphocytes (green), monocytes (blue) and high scatter (red) events can be used to orient these populations on the eccentricity vs radial moment plot.
Chat with us about how the BD FACSDiscover™ instruments will elevate your lab
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