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Real Yellow/Real Blue Family Campaign

BD Horizon RealYellow™ and RealBlue™ Reagents

The superior alternative to PerCP and PE tandem dyes

 

Reduced Monocyte Background

Reduced Monocyte 
Background

Reduced Spillover

Reduced Spillover

Intracellular Staining

Intracellular Staining

reduced monocyte background

RealYellow™ and RealBlue™ Reagents Offer Decreased Monocyte Background Compared to PE-Cy Tandems

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BD Horizon™ RB780 Reagents offer reduced background compared to PE-Cy7

RY775-Improved-nonspecific-Binding
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BD Horizon™ RY775 Reagents provide improved nonspecific binding

reduced spillover

RealYellow™ and RealBlue™ Reagents Offer Reduced Spillover Compared to PE and PerCP Tandems

RB610 Reduced Spillover Compared to PE Dazzle and PE CF594
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BD Horizon™ RY610 Reagents show reduced spillover into the corresponding blue detectors.

RB705 Reduced Spillover Compared with PerCP and CY5.5
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BD Horizon™ RB705 Reagents show reduced spillover into the corresponding yellow-green detectors.

RY586 Reduced Spillover Compared with PE Tandem Dyes
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BD Horizon™ RY586 Reagents offer high resolution with minimal cross-laser excitation. Data was acquired on a BDFACSymphony™ A5 SE Cell Analyzer.

Intracellular marker detection

RealYellow™ and RealBlue™ Reagents Offer the Detection of Low-expression Surface and Intracellular Markers.

RY703-Reagent-Lower-Expression-Intracellular-Markers
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BD Horizon™ RY703 Reagent resolves low-expression intracellular markers

Cytokine-Marker
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BD Horizon™ RB780 Reagents support the detection of low-expression intracellular markers.

RY775-Resolves-Intracellular-Markers
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BD Horizon™ RY775 Reagent resolves intracellular markers

Dye Summary Table

Switch to Cleaner BD Horizon™ Real Dyes

FormatSpectralConventionalRelative BrightnessSpillover
(1-low, 4-high)		Alternative ToProducts
Blue Laser
RB545✓‒ 1(Unique position)View
RB575✓✓ 1PE, StarBright™ Blue 580View
RB613✓✓ 2PE-CF594, PE-Dazzle™ 594, BB630-P2, StarBright™ Blue 615View
RB670✓✓ 2PE-Cy5, BB660-P2, StarBright™ Blue 675View
RB705✓✓ 2PerCP-Cy5.5, BB700, StarBright™ Blue 700View
RB744✓✓ 1BB755-PView
RB780✓✓ 1PE-Cy7, BB790-PView
RB824✓✓ 1PerCP/Fire™ 806, StarBright™ Blue 810View
Yellow-Green Laser
RY586✓✓ 1PEView
RY610✓✓ 1PE-CF594, PE-Dazzle™ 594View
RY655✓✓ 3PE-Cy5, StarBright Yellow 665View
RY703✓✓ 2PE-Cy5.5, StarBright™ Yellow 720View
RY743✓✓ 2PE/Fire™ 744View
RY775✓✓ 2PE-Cy7, StarBright™ Yellow 800View


Chart contains representative fluorochromes compatible with a 5-laser spectral flow cytometer. Table may differ based on instrument configuration and settings. Spillover ranking is based on cross-laser excitation and does not take into account spillover into adjacent or residual detectors. PE and PE tandem dyes are excited by both the blue and yellow-green lasers. Be sure to check your instrument configuration.

Visit the BD Horizon™ Real Dyes  page to learn more about these fluorochromes and others in the family

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